丁酸梭菌及荔枝多酚联合应用对腹泻小鼠抗氧化功能的影响

Effect of Combined Application of Clostridium butyricum and Lichee Polyphenols on Antioxidant Function of Diarrhea Mice

探讨丁酸梭菌和荔枝多酚联合应用对腹泻小鼠机体氧化还原状态的调节功能。设空白组、模型组、荔枝多酚组、丁酸梭菌组以及丁酸梭菌—荔枝多酚混合液低、中、高剂量组,于试验第1天至第10天分别进行预防性及治疗性给药,试验第5天时,利用番泻叶水煎剂灌胃法制备小鼠腹泻模型,试验第10天时,对各组小鼠血清中的MDA含量、SOD活性、GSH含量和小肠组织MPO活力等指标进行测定。结果表明,腹泻模型小鼠抗氧化能力降低,小肠组织发生炎症;不同浓度的丁酸梭菌—荔枝多酚混合液能够不同程度地降低腹泻小鼠体内的氧化产物MDA含量,提高还原酶SOD活性,促进还原性物质GSH生成,同时可以减轻炎症反应,其中以混合液中剂量组效果最优。综上提示,丁酸梭菌与荔枝多酚联合应用可提高腹泻小鼠的抗氧化能力,二者具有协同效应。

This study aimed to evaluate the regulative effects of combined use of Clostridium butyricum and lichee polyphenol on redox state of mice. The blank group, diarrhea model group, lichee polyphenol group, Clostridium butyricum group and low,moderate and high dosage of lichee polyphenol and Clostridium butyricum mixture groups were included in present study. From the 1stto 10 thday of the experimental period, the mice were administered for prevention and therapy purpose. At the 5thday, the diarrhea model in mice was prepared by intragastrical administration of folium sennae decoction. At the 10 thday, the serum MDA content, SOD activity, GSH content and MPO activity in intestinal tissue were assessed. The results revealed that the antioxidant capacity of the diarrhea mice was reduced and the inflammation in intestinal tissue was observed; The different dosages of lichee polyphenol and Clostridium butyricum mixture was able to reduce the content of oxidation products MDA, improve the activity of reductase SOD, stimulate the generation of reducing substances GSH, and inhibit the inflammation. The optimum effect was observed in moderate dosage group. It is demonstrated that the combined application of Clostridium butyricum and lichee polyphenols was able to improve the antioxidant capacity of the diarrhea mice and they have synergistic effect.