不同乳酸菌分离株抗菌物质及其抗菌活性的体外检测及鉴定

In vitro analysis of substance with antibacterial activity of lactic acid bacteria

为检测及鉴定乳酸菌分离株的抗菌物质,本研究采用牛津杯法测定乳酸菌的抗菌活性,通过上清液煮沸、过氧化氢排除试验、蛋白类排除试验、有机酸排除试验检测并鉴定了本实验室前期从健康奶牛阴道内分离获得的5株酸菌的抗菌物质。结果显示,这5株乳酸菌对指示菌均具有良好的抗菌活性。坚强乳杆菌、粪肠球菌、屎肠球菌和巴黎链球菌的主要抗菌物质为有机酸,黏膜乳杆菌的抗菌物质为过氧化氢、蛋白和有机酸。本研究表明黏膜乳杆菌可以初步作为防治奶牛子宫内膜炎的潜在益生菌使用。

To identify the antibacterial substance of lactic acid bacteria, the substance from lactic acid bacteria was identified and tested by Oxford cup method, i.e. the antibacterial substances of lactic acid bacteria were analyzed by boiling the bacteria culture medium, protease inhibition, hydrogen peroxide and organic acid inhibition. The results show that 5 lactic acid bacteria isolated from healthy dairy cow vaginas in our previously study had good antibacterial activity against indicator bacteria. The major antibacterial substance of Enterococcus durans, Enterococcus faecalis, Enterococcus faecium and Streptococcus lutetiensis was organic acid, and the antibacterial substance of Lactobacillus mucosae was hydrogen peroxide, protein and organic acid. These data indicated that the L.mucosae was more efficient to inhibit the bacteria, which could be preliminary used as probiotic to prevent and treat dairy cow endometristis.