艾灸对AD大鼠学习记忆能力及海马Aβ、IL-1β、IL-2表达的影响

Effect of Moxibustion on the Learning and Memory of Rat Models of Alzheimer's Disease and the Expression of Hippocampal Aβ, IL-1β and IL-2

目的观察艾灸对阿尔茨海默病(Alzheimer’s Disease,AD)模型大鼠学习记忆能力及海马β淀粉样蛋白(amyloid β protein,Aβ)1-42、白细胞介素1β(interleukin-1β,IL-1β)和白细胞介素-2(interleukin-2,IL-2)表达,探讨艾灸治疗AD的作用及其机制。方法将48只健康SD雄性大鼠随机分为假手术组、模型组、西药组和艾灸组,每组12只。采用双侧侧脑室注射链脲佐菌素(streptozotocin,STZ)制备AD大鼠模型。术后第10天起,艾灸组采用艾灸百会、命门、长强及关元穴治疗,同时西药组给予盐酸多奈哌齐灌胃治疗。治疗30 d后应用水迷宫测试检测各组大鼠的学习记忆能力的变化,采用免疫组织化学方法检测各组大鼠海马Aβ1-42的情况,并采用双抗体夹心法检测各组大鼠海马区IL-1β以及IL-2的表达。结果与模型组比较,艾灸能显著减少海马中Aβ42沉积的情况(P<0.05),降低IL-1b蛋白水平(P<0.05),提高IL-2蛋白水平(P<0.05),并且显著改善模型大鼠的学习记忆能力(P<0.05)。结论艾灸能提高机体的免疫力和学习能力,其机制可能与降低海马中IL-1β含量、升高IL-2含量以及改善Aβ42沉积有关。

Objective To observe the effect of moxibustion on the learning ability and memory of rat models of Alzheimer＇s disease（AD） and the expression of amyloid β protein（Aβ）-42, interleukin（IL）-1β, and IL-2, for unveiling the function and mechanism of moxibustion in treating AD. Method Forty-eight healthy male Sprague-Dawley rats were randomized into a sham-operation group, a model group, a Western medication group, and a moxibustion group, 12 rats in each group. The AD model rats were established by injection of Streptozotocin（STZ） into bilateral ventricles. From the 10 th day after the operation, the moxibustion group started to receive moxibustion at Baihui（GV 20）, Mingmen（GV 4）, Changqiang（GV 1）, and Guanyuan（CV 4）; meanwhile, the Western medication group received Donepezil hydrochloride via intragastric administration. After 30-day treatment, the learning and memory ability was tested by using water maze, the hippocampal Aβ-42 was examined by immunohistochemical method, and the expression of hippocampal IL-1β and IL-2 was by double-antibody sandwich ELISA. Result Compared to the model group, moxibustion significantly down-regulated the levels of Aβ-42（P〈0.05） and IL-1β protein（P〈0.05）, up-regulated the level of IL-2 protein（P〈0.05） in hippocampus, and markedly improved the learning and memory of AD rats（P〈0.05）. Conclusion Moxibustion can enhance the immunity and learning ability, which is plausibly related to the down-regulation of IL-1β, up-regulation of IL-2, and improvement of Aβ-42 in hippocampus.