大鼠创伤性脑损伤后精神障碍的精神行为学改变

Spirit Behavioral Changes of Mental Disorders Caused by Traumatic Brain Injury in Rats

目的利用改良FEENEY’S法建立大鼠创伤性脑损伤(TBI)模型,探讨TBI后精神障碍的精神行为学改变。方法将成年雄性SD大鼠随机分为正常对照组、TBI组和假手术组,每组各10只。采用改良FEENEY’S法建立大鼠TBI模型。TBI后15 d开始,分别依次进行旷场实验(OFT)和强迫游泳实验(FST)。记录OFT中大鼠5 min内的移动总距离(DMT)、平均速度(VM),记录FST中大鼠5 min内不动时间总和(ITT),观察大鼠的高警觉性、焦虑/抑郁和恐惧行为的变化。结果 TBI组大鼠的DMT[(1 228.10±204.97)cm]、运动VM[(4.09±0.68)cm/s]较假手术组[(1 660.01±332.09)cm、(5.53±1.10)cm/s]和正常对照组[(2 022.32±280.92)cm、(7.28±1.80)cm/s]均明显减少,差异均有统计学意义(P<0.05)。TBI组大鼠的ITT[(149.88±27.36)s]较假手术组[(120.55±22.31)s]、正常对照组[(106.10±29.03)s]明显增加,差异有统计学意义(P<0.05)。结论 TBI后,大鼠出现了焦虑/抑郁样精神行为异常。

Objective To set up traumatic brain injury（TBI）in rats by the improved device of FEENEY＇s weight dropping model and to explore the spirit behavioral changes of mental disorders caused by TBI. Methods SD rats were randomly divided into normal control group（normal group,n = 10）,traumatic brain injury group（TBI group,n = 10）and sham group（n = 10）. The improved device of FEENEY＇s method was adopted to establish rat TBI model. For the following 15 days after TBI,open-field（OF）and forced swimming（FS）tests were carried out and the behavior of the TBI rats within 5 minutes were recorded,the behavioral indexes,such as distance moved total（DMT）,velocity mean（VM）and immobility time total（ITT）,were analyzed. With above experiments,rats＇ fear response to environment,high alertness and anxiety and depression behavior were evaluated. Results DMT and VM of TBI group rats（1 228.10±204.97 cm,4.09±0.68 cm/s）were significantly lower than those of the normal group（2 022.32±280.92 cm,7.28±1.80 cm/s）or sham group（1 660.01±332.09 cm,5.53±1.10 cm/s）（P〈0.05）.The ITT of TBI group（149.88±27.36 s,P〈0.05）was longer than that of the normal group（106.10±29.03 s）or sham group（120.55±22.31 s）significantly（P〈0.05）.Conclusion TBI leads to rats＇ abnormal emotional and behavioral response.