DEK蛋白在膀胱尿路上皮癌癌组织和尿液中的表达及临床意义

Expression of DEK Protein in the Tissue and Voided Urine of Patients with Bladder Urothelial Carcinoma and Its Clinical Significance

目的探究DEK蛋白在膀胱尿路上皮癌癌组织和尿液中的表达及其与肿瘤病理分期之间的关系。方法应用免疫组化技术检测48例膀胱尿路上皮癌组织和18例癌旁正常组织DEK蛋白的表达,应用蛋白免疫印迹的方法检测28例膀胱尿路上皮癌患者和6例健康者(对照组)尿中的DEK蛋白的表达。结果膀胱尿路上皮癌癌组织中DEK蛋白的阳性表达率(73%)高于癌旁正常组织(33%,P<0.05);浅表性膀胱尿路上皮癌组织中DEK的阳性表达率(86%)高于浸润性(55%,P<0.05);膀胱尿路上皮癌患者尿液中DEK相对表达量(0.834)明显高于对照组(0.242,P<0.05);以对照组尿液中DEK的相对表达量作为标准,诊断膀胱尿路上皮癌的灵敏度可达82.1%。结论 DEK蛋白在膀胱尿路上皮癌患者癌组织和尿液中呈现阳性表达,与膀胱癌的病理分级有密切关系。癌症早期DEK蛋白在组织中的阳性表达高于癌症晚期。组织中和尿液中DEK表达认为是潜在的诊断膀胱尿路上皮癌的肿瘤标记物。

Objective To detect the presence of DEK protein in the tissue and voided urine of patients with bladder urothelial carcinoma,and analyze the relationship between DEK protein and clinical pathological classification of bladder cancer. Methods The expression of DEK protein was examined by immunohistochemistry in 48 bladder cancer tissues and 18 adjacent normal tissues（control group）. The presence of DEK protein in voided urine was analyzed by western blot in 28 bladder cancer patients and 6 healthy individuals（control group）. Results The positive expression of DEK protein in bladder tumor tissues（73%）is higher than in adjacent normal tissues（33%,P〈0.05）. The expression of DEK in superficial bladder cancer（86%）was found higher than invasive bladder cancer（55%）with significant differences（P〈0.05）. The expression of DEK in urine samples of bladder cancer patients（0.834）was found higher than control group（0.242,P〈0.05）;Compared with the DEK expression of control group,the sensitivity of diagnosis of bladder cancer with voided urine could be 82.1%. Conclusion DEK protein positive expressed in tissues and voided urine of patients with bladder urothelial carcinoma. The expression was correlated with pathological classification of bladder cancer. The positive rate of DEK expression in early stage of tumor tissues is higher than late stage. The presence of DEK protein in tissues and voided urine can be considered as a suitable biomarker for bladder cancer potentially.