两系不育系Y58S的花药培养与花培后代的鉴定筛选

Primary research on anther culture of photo-thermosensitive genic male sterile rice “Y58S” and selection of descendant of anther culture

花药培养技术与传统不育系育种的结合能有效加快新的光温敏不育系材料的选育进程。为了筛选出适合两系不育系Y58S的花药培养条件,利用10种不同培养基对两系不育系Y58S不同杂交组合F2代进行花药培养。培养基E(NMB+2mg/L2,4-D+2mg/LNAA+1mg/LKT+0.5g/L脯氨酸+0.5g/L谷氨酰胺+0.5g/L水解酪蛋白+25g/L蔗糖+25g/L麦芽糖+10g/L琼脂粉)较为适合两系不育系Y58S的花药培养;同时,材料的基因类型对花药培养力有着显著的影响,粳稻血缘的渗入有利于两系不育系花药培养力的提高。花药培养后代田间性状观察结果表明,在当代花药培养植株中发现较多的二倍体植株,少部分为单倍体、四倍体植株,尚未发现非整倍体;筛选出一个表现较好的不育株系。

The combination of anther culture techniques and traditional sterile lines breeding can effectively speed up the improvement of photo-thermosensitive genic male sterile（PTGMS） lines. To screen for the optimal culture medium for the two-line male sterile line, Y58S,we studied the effects of 10 different culture mediums on the F2 generation of different cross combination between Y58S and selected male parents. The results showed that medium E（NMB＋2 mg/L 2,4-D ＋2 mg/L NAA＋1 mg/L KT＋0.5 g/L Pro＋0.5 g/L Gln＋0.5 g/L CH＋25 g/L sugar＋25 g/L Maltose＋10 g/L Agar）was more effective for anther culture of PTGMS lines. And introducing japonica rice by cross breeding improved anther culture ability of PTGMS lines,indicating that genetype played an important role in rice anther culture. The examination on traits in field of descendants displayed that most of the plants were diploid, some were haploid and tetraploid, and no non-integer ploidy,and a new PTGMS lines was screened.