水稻抗二化螟特性鉴定技术的评价研究

Evaluation of Appraisement Method for Rice Resistance to Striped Stem Borer

采用离体叶片、离体茎秆、离体剥开茎秆及活体成株4种室内抗虫性鉴定方法,对相同水稻材料进行抗二化螟特性鉴定,同时对这些水稻室内抗二化螟鉴定技术进行了综合评价。结果表明,这些方法的抗性鉴定结果高度吻合,离体叶片鉴定方法是目前最快速、有效的抗二化螟鉴定方法。并通过离体叶片和活体成株鉴定方法对转Vip3A基因水稻材料进行鉴定,再次验证了离体叶片鉴定方法的有效性。此外,初步发现对于同为鳞翅目水稻害虫稻纵卷叶螟,用离体叶片鉴定方法也可进行有效鉴定。

The identification result was consistent with the resistance evaluation to striped stem borer through four different bioassay methods, including detached-leaf bioassay, detached-stem bioassay, stripping detached-stem bioassay and whole plant bioassay. The detached-leaf bioassay is considered as the most rapid and effective method for evaluating resistance ability to striped stem borer. By both detached-leaf and whole plant bioassays, the author identified transgenic rice with Vip3 A gene, and further validated the identification effectiveness of the detached-leaf bioassay method. Simultaneously, our preliminary results revealed that detached-leaf bioas-say could also perform an accurate identification to rice leaf roller as lepidoptera pests of rice.