辣椒白绢病病原菌的分离与鉴定

Separation and Molecular Identification of Southern Blight Pathogen in Hot Pepper

辣椒白绢病为辣椒土传病害,病原菌为齐整小核菌(Sclerotium rolfsii)。为准确鉴定辣椒白绢病,在江苏南京地区采集到疑似辣椒白绢病病株,采用常规植物病原菌分离方法,分离菌核H样和菌丝S样,经PCR扩增和BLAST比对,确定病原菌为齐整小核菌。通过初步建立分子检测技术体系,以期为江苏地区辣椒白绢病的对症用药提供技术支撑。

Hot pepper southern blight is a soil-borne disease, and its pathogen is Sclerotium rolfsii. In order to accurately identify southern blight in hot pepper, some suspected hot pepper plants infected with southern blight were collected from Nanjing, Jiangsu Province, and used with conventional phytopathogen separation methods to separate sclerotial sample H and mycelial sample S and identify the pathogen as Sclerotium rolfsii through PCR amplification and blast alignment in this study. The molecular detection technology system has been established so as to provide technical support for the Sclerotium rolfsii proper control in Jiangsu.