CRTH2^+CD4^+T细胞在特应性皮炎儿童外周血中的表达和意义

Expression and significance of CRTH2^+CD4^+T cells in peripheral blood of children with atopic dermatitis

探讨CRTH2^+CD4^+T细胞在特应性皮炎(atopic dermatitis,AD)儿童外周血中的比例变化及意义。选取2015年6月至2015年10月间我院皮肤科门诊就诊的AD患儿22例和同龄正常对照组儿童30例,采用流式细胞术检测两组儿童外周血中CRTH2^+CD4^+T细胞的比例和该细胞分泌的IL-4、IFN-γ水平;采用粉尘螨刺激PBMC,利用CCK-8增殖试验结合流式细胞术检测两组CRTH2^+CD4^+T细胞的比例变化。结果显示,AD组儿童外周血中CRTH2^+CD4^+T细胞比例明显高于对照组(P<0.05),且在AD组中这一比例大小与血清IgE浓度呈正相关(r=0.602,P<0.05);CRTH2^+CD4^+T细胞主要分泌IL-4,基本不分泌IFN-γ;粉尘螨刺激后,PBMC中CRTH2^+CD4^+T细胞比例升高,并且AD组的刺激前后细胞比例差值明显高于对照组(P<0.05),提示CRTH2^+CD4^+T细胞参与了儿童特应性皮炎的发病过程。

To explore the characteristic, percentage and clinical significance of CRTH2＋ CD4＋ T cells in peripheral blood of children with atopic dermatitis, 22 children with atopic dermatitis in our clinic and 30 healthy children were selected. The per- centage and attributes of CRTH2＋ CD4＋ T cells in peripheral blood were measured by flow cytometry. CCK-8 assay combined with flow cytometry were used to analyze the change in the percentage of CRTH2＋ CD4＋ T cells from PBMC after dust mites stimulation. The average percentage of CRTH2＋ CD4＋ T cells in peripheral blood in AD group was higher than that in the control group（P 〈0. 05）, and there was a positive correlation between serum IgE level and the percentage of CRTH2＋ CD4＋ T cells（r = 0. 602,P 〈0. 05）, CRTH2＋ CD4＋ T cells secretod high amounts of IL-4 , but not IFN-γ. The percentage of CRTH2＋ CD4＋ T cells in PBMC after dust mites stimulation increased, and the average gap percentage of CRTH2＋ CD4＋ T cells in AD group was more dominant than that in control group（P 〈0, 05）, indicating that CRTH2＋ CD4＋ T cells may play an important role in the pathogenesis of children＇s atopic dermatitis.