ICOS/ICOSL、OX40/OX40L及CD40/CD40L在系统性红斑狼疮患者外周血表达水平及其临床意义

Study on ICOS/ICOSL,OX40/OX40L and CD40/CD40L in the periphery blood of patients with system lupus erythematosus and their clinical significance

本研究旨在检测SLE患者外周CD4+T细胞表面ICOS、OX40、CD40L及B细胞表面ICOSL、OX40L、CD40分子的表达情况,并探索其临床意义。采用流式细胞术,我们检测SLE患者及健康对照外周CD4+T细胞表面ICOS、OX40、CD40L及B细胞表面ICOSL、OX40L、CD40分子的表达,并分析其与SLE临床指标的相关性。结果显示,SLE患者外周CD4+T细胞上ICOS、OX40、CD40L及B细胞上ICOSL、OX40L、CD40分子的表达均明显高于健康对照组(均P<0.05),且SLE患者CD4+T细胞表面ICOS的表达与患者血清IgG水平呈现正相关,B细胞表面ICOSL的表达与患者血清抗dsDNA抗体水平正相关,B细胞表面OX40L的表达与患者血清抗核抗体水平正相关(均P<0.05)。上述结果表明SLE患者外周CD4+T细胞上ICOS、OX40、CD40L及B细胞上ICOSL、OX40L、CD40分子均发生上调,并与SLE自身抗体水平呈现正相关,提示分别表达于T细胞和B细胞表面的ICOS/ICOSL、OX40/OX40L及CD40/CD40L分子,通过相互作用参与SLE的致病过程。

To investigate the role of ICOS/ICOSL, OX40/OX40L and CD40/CD40L in systemic lupus erythematosus（SIzE）, we have compared ICOS, OX40, CD40L expressions on the surface of CD4＋ T cells and ICOSL, OX40L, CD40 on B cells between SLE patients and healthy controls by flow cytometry. The elinical significance of them was further analyzed. The results indi cared that both ICOS, OX40, CD40L on CD4＋ T cells and ICOSL, OX40L, CD40 on B cells were up-regulated in SLE patients when compared with healthy eontrols（P 〈0.05）. In the patients, we found that the expression level of ICOS on CD4＋ T cells positively correlated with IgG levels （P 〈0.05） , whereas, the expression level of ICOSL on B cells positively correlated with anti-dsDNA levels （P 〈0.05）. In addition, The expression level of OX40L on B cells positively correlated with ANA levels （P 〈0.05）. Taken together, our results demonstrate that ICOS/ICOSL, OX40/OX40L and CD40/CD40L were up-regulated on the peripheral lymphocytes of SLE patients with significant correlation to disease severity and autoantibody production. The in teraction between ICOS/ICOSL, OX40/OX40L and CD40/CD40L might mediate important roles in the pathogenesis of SLE.