不同保存期的去白细胞悬浮红细胞上清液中微粒粒径分布研究

The Particle Size Distribution in Leukocyte-reduced Red Blood Cell Suspensions in Different Durations of Sample Storage

目的探索库存去白细胞悬浮红细胞（去白悬浮红）随着保存时间的延长,红细胞破损或溶血而产生的细胞碎片以及微粒变化,为输血安全提供启示性实验基础。方法取不同保存天数（3、7、14和21 d）的库存去白悬浮红进行上清液制备,应用动态光散射仪对样品中的各种微粒进行粒径分布分析。结果 3 d和7 d的库存去白悬浮红上清液样品中的微粒主要为10-25 nm微粒;从14 d开始,上清液样品中出现了更多150-300 nm的微粒,到21 d时,150-300 nm范围内的微粒占绝大部分。结论可通过测定库存红细胞制品上清液中微粒的粒径变化来评估红细胞的储存损伤程度,进一步探讨新的溶血评价方法。

Objective As storage time extension, fragments and particles changes need to be explored for breakage and hemolysis of aleukocytic red blood cell suspensions, so as to provide a experimental basis for the transfusion safety. Methods Aleukocytic suspending red blood cells from stocks were prepared in different storage days（3d, 7d, 14 d and 21d）. Particles in the samples according to the grain size distribution were analyzed through dynamic light scattering.Results Particles with the size from 10 to25 nm in the supernatant of the leukocyte-free red blood cells on 3d and 7d, while particles of 150~300 nm in size were predominantly noted after 3 weeks storage. Conclusion Particle size measurements in the RBC suspension of leukocyte reduced storage samples would be useful for evaluation the hemolysis in RBC transfusion.