利用SSR标记鉴定江苏水稻品种真实性的研究

Authenticity Identification of Rice Varieties Planted in Jiangsu Province by SSR Markers

利用93个SSR标记对2015年江苏水稻种子市场中的355份抽检样品的真实性进行鉴定,与标准样品间存在2个及以上位点差异的占比31.55%。发现与标样不存在或仅存在1个位点差异的抽检样品,其与标样在农艺性状上不存在差异;而存在2个及以上位点差异的抽检样品中,随着差异位点数增多,差异明显增多。判定64个抽检样品的真实性存在问题,占抽样品种的18.03%。发现单独使用农业行业标准中的或之前报道的SSR标记,均不能完全鉴别江苏水稻种子市场中的假冒套牌品种,而采用2套标记整合后的93个标记则可完全鉴别。

A total of 93SSR（Simple sequence repeat）markers were employed to identify the authenticity of rice varieties randomly sampled from rice seed market of Jiangsu Province in2015.Compared with the standard varieties,up to 31.55% samples were found difference on more than 2SSR loci.We found that the samples with less than 1different SSR loci all displayed same morphological phenotype as the corresponding standard variety;while,for the samples with more than 2different SSR loci,we found that there was significant increase in the probability of morphological difference as the number of different loci rose.A total of 64 samples were screened out with the problem on variety authenticity,which was about 18.03%in total samples.Neither of the two sets of SSR markers could distinguish all these varieties.The combination of the two sets of 93 markers altogether could easily distinguish them.