摘要
利用AFLP和SSR分子标记技术,对甘肃省的16个马铃薯主栽品种进行了遗传多样性分析,结果表明：在AFLP标记分析中,16个马铃薯品种间的遗传距离介于0.244 9~0.649 6,平均值为0.401 2;SSR标记分析中,16个马铃薯品种间的遗传距离介于0.135 9~0.801 4,平均值为0.477 0。AFLP标记具有丰富的多态性和较高的检测效率,SSR标记具有共显性、重复性好、扩增结果稳定等特点,两种技术均被应用于马铃薯品种遗传多样性分析、遗传图谱构建和基因定位等方面。
In this paper,the AFLP and SSR marker are used to study the genetic diversity of 16 potato cultivals in Gansu province. The study shows that the analysis of AFLP marker the genetic distance of 16 potato cultivars is from 0.244 9 to 0.649 6 with average value being 0.401 2,and the genetic distance of 16 potato cultivars is between 0.135 9~0.801 4 with average valuebeing 0.477 0.AFLP markers with rich polymorphism and high detection efficiency,and SSR markers withcodominant,good repeatability,and stable amplification results are applied to the analysis of genetic diversity,genetic map construction,gene mapping.
出处
《甘肃农业科技》
2016年第7期1-6,共6页
Gansu Agricultural Science and Technology
基金
国家自然基金(31160299)
甘肃省青年科技基金计划(1308RJYA047)
甘肃省农业科学院农业科技创新专项(2013GAAS43)
