条斑紫菜不同栽培品系的遗传多样性分析

Genetic Diversity of Different Cultivars of Pyropia yezoensis

【目的】确定通过杂交和突变选育出的条斑紫菜（Pyropia yezoensis ）栽培品系的遗传多样性。【方法】采用简单重复序列区间（Inter simple sequence repeat,ISSR）、扩增片段长度多态性（Amplified fragment length pol-ymorphism,AFLP）和随机扩增多态性DNA（Random amplified polymorphism DNA,RAPD）等3种分子标记对条斑紫菜不同栽培品系进行遗传多样性分析。【结果】3种分子标记的多态性比率分别为38.10%、38.95%和61.75%；所表现出来的不同栽培品系间遗传距离分别为0.1785,0.1029和0.2845；3种分子标记聚类结果并不完全一致,但可以看出 Y-0602、Y-HA01和 Y-H001总是聚类在一起,而 Y-DL02倾向于单独聚为一支。【结论】通过诱变和杂交所选育的品系,在遗传角度上仍然属于条斑紫菜,但已形成不同栽培品系,具有一定的遗传多样性。

Objective]Different cultivars were screened and bred by mutation or hybridization, and their genetic diversity was determined.[Methods]Molecular markers ISSR,AFLP and RAPD were employed to analyze the genetic diversity of screened cultivars.[Results]The poly-morphism ratio shown by ISSR,AFLP and RAPD was 38.10%,38.95% and 61.75%,respec-tively.The genetic distance shown by ISSR,AFLP and RAPD between different cultivars of Pyropia yezoensis was 0.1785,0.1029 and 0.2845,respectively.Although the clustering results were not consistent,the cultivars Y-0602,Y-HA01 and Y-H001 always clustered to-gether,whereas Y-DL02 tended to cluster as outgroup.[Conclusion]After being screened and bred by mutation or hybridization,the cultivars studied in the present paper are genetically the same species as P.yezoensis but essentially dif-ferent cultivars with certain genetic diversity.