复方麦芽丸对多囊卵巢综合征模型大鼠3-β-羟甾脱氢酶、细胞色素P450酶表达的影响

Effects of compound malt pill on expression of 3- β- HSD and CYP450 in polycystic ovary syndrome model rats

目的研究复方麦芽丸(CMP)对多囊卵巢综合征(PCOS)大鼠3-β-羟甾脱氢酶(3-β-HSD)、细胞色素P450酶(CYP450)表达的影响,探讨其可能的作用机制。方法 9日龄SD大鼠于颈背部皮下注射睾丸酮建立PCOS模型,随机分为模型组,阳性对照组和CMP低、中、高剂量组(分别相当于丸药量3.15、6.3、12.6 g·kg^(-1))(n=9),另设正常组(n=10)。CMP低、中、高剂量组按10 m L·kg^(-1)分别灌以相应浓度的中药煎剂,阳性对照组灌以炔雌醇环丙孕酮片水溶液,正常组和模型组均灌以等量的蒸馏水。观察大鼠动情周期及卵巢大体形态变化,运用免疫组化、Western blot、RT-q PCR测定卵巢组织3-β-HSD、CYP450的蛋白和m RNA表达。结果 CMP可以降低PCOS模型大鼠3-β-HSD表达水平(P<0.01),升高模型大鼠CYP450表达水平(P<0.01),均以高剂量组的作用效果最佳。结论 3-β-HSD和CYP450共同参与了PCOS模型大鼠卵巢的激素调节;CMP治疗PCOS的机制可能是通过调节卵巢组织3-β-HSD、CYP450的表达水平实现的。

AIM To observe the effect of Chinese medicine compound malt pill （CMP） on the expression of 3- β- hydroxysteroid dehydrogenase （3-β- HSD） and cytochrome P- 450 enzyme system （CYP450） in rat model of polycystic ovary syndrome （PCOS）, and to further explore its possible mechanism. METHODS Female SD rats of 9-day old were injected subcutaneously with testosterone propionate for inducing PCOS model.The model rats were randomly divided into 5 groups： model group, positive control group, and high- , moderate- and low-dosage CMP group （equivalenting to pills of 3.15, 6.3, 12.6 g.kg-~）, 9 in each group. Ten rats with normal estrus cycles were served as the normal control. CMP dose groups were given the corresponding concentrations of traditional Chinese medicine decoction according to 10 mL-kg-1, the positive control group was given aqueous solution of ethinylestradiol and cyproterone acetate tablets, and the normal group and the model group were given distilled water. The estrus cycles and ovary morphology changes were observed, and the expression levels of 3-β-HSD and CYP450 were detected by immunohistochemical, Western blot and RT-qPCR methods. RESULTS The CMP could decrease the expression of 3- [3- HSD （P 〈 0.01） and up-regulate the expression of CYP450 （P 〈 0.01） in the PCOS model rats, with the best effect in high dosage of the CMP group. CONCLUSION 3-[3-HSD and CYP450 may participate in the ovarian hormonal regulation in the PCOS rats. CMP shows therapeutic efficacy on PCOS rats through regulating expression level of 3-β-HSD and CYP450 in ovarian tissue.