山丹黄参多糖对四氯化碳致小鼠急性肝损伤的影响

Effects of Shandan Sphallerocarpus racills Polysaccharide on Urgent Hepatic Injury Induced by CCl_4 in Mice

为了研究山丹黄参多糖对四氯化碳(CCl4)致小鼠(Mus musculus)急性肝损伤及相关酶活性的影响,对50只小鼠分别灌胃0.2 ml山丹黄参多糖(12.5、25.0、37.5 g/L)或生理盐水7 d,最后一次灌胃1 h后腹腔注射1%的CCl4橄榄油溶液0.2 ml,16 h后处死小鼠,比色法检测血浆丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活力的变化,显微镜观察肝组织结构的变化,免疫组织化学法检测肝组织中转化生长因子-β_1(TGF-β_1)表达的变化。与正常对照组相比,实验对照组小鼠体重减轻,血浆丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)活力显著升高(P<0.01),肝明显肿胀,肝组织结构不清,肝细胞出现炎性坏死,转化生长因子-β_1(TGF-β_1)阳性表达明显增强(P<0.01)。与实验对照组相比,山丹黄参多糖各组小鼠体重增加,血浆丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)活力明显降低(P<0.01),肝无明显肿胀,肝索清晰,炎性坏死很少,细胞结构清晰,转化生长因子-β_1阳性表达明显减少(P<0.01)。表明一定剂量的山丹黄参多糖能增强机体活力,促进机体新陈代谢,降低正常细胞的凋亡,对四氯化碳造成的肝组织损伤有一定的保护作用。

To investigate the effect of Shandan Sphallerocarpus racills polysaccharide（SRP） on urgent hepatic injury induced by carbon tetrachloride（CCl4）, 50 mice（Mus musculus） were gavaged with 0.2 ml SRP at different concentrations（12.5, 25.0, 37.5g/L） or physiological saline for 7 days, then intraperitoneally injected with 0.2 ml CCl4 solution（1% in olive oil） at the last day, and sacrificed 16 h later. The morphological changes of hepatic tissues were observed under microscopy, the activities of plasma alanine aminotransferase（ALT） and aspartate aminotransferase（AST） were determined by colorimetry, the expression change of transforming growth factor-β1（TGF-β1） in liver tissue were determined by immunohistochemical method.The data were processed with spss 13.0. The results were expressed in Mean ± SD. Compared to the natural control group（saline group）, the body weights were decreased（Table 1）, activities of plasma ALT and AST were significantly increased（P〈0.01）（Table 2）,the liver tissue structure was in clear tumefaction,liver cells were necrotic and seriously vacuolated（Fig. 1）, and the expression of TGF-β1 was significantly increased（P〈0.01） in the experimental control group（CCl4 treatment group）. However, the body weights were significantly increased（Table 1）, the activities of plasma ALT and AST were significantly decreased（P〈0.01）（Table 2）, the liver showed no obvious swelling, hepatic cord was clear, inflammatory necrosis was rare, the liver celluar structure was clear（Fig. 1）, and the expression of TGF-β1was decreased significantly（P〈0.01） in the SRP groups. These data indicate that SRP may enhance the cellular activity, accelerate metabolism, and reduce cellular apoptosis, and protect liver damage induced by carbon tetrachloride in mice.