不同浓度丙泊酚通过缝隙连接通讯对舒张大鼠肠系膜动脉的影响

Propofol relaxes mesenteric arteriole via gap junction

目的观察丙泊酚对SD大鼠肠系膜动脉的影响和对肠系膜动脉舒张作用是否与缝隙连接通讯相关。方法应用压力肌动图技术,在急性分离的SD大鼠肠系膜动脉2级血管上,观察1×10-7、3×10-7、1×10-6、3×10-6、1×10-5、3×10-5、1×10-4和3×10-4 mol/L丙泊酚对血管直径的影响,预孵育缝隙连接阻断剂18-β甘草次酸(18β-glycyrrhetinic acid,18β-GA)和2-aminoethoxydiphenylborate(2-APB)后,观察丙泊酚对血管直径的影响。结果给予1×10-7、3×10-7、1×10-6、3×10-6、1×10-5、3×10-5、1×10-4和3×10-4 mol/L的丙泊酚,使SD大鼠血管直径从(208.6±13.4)μm分别增加至(213.5±13.6)、(219.7±13.2)、(226.4±12.5)、(234.9±12.3)、(245.5±13.0)、(267.4±15.2)、(336.2±18.3)和(385.9±14.2)μm,1×10-4 mol/L的丙泊酚引起血管直径随着丙泊酚浓度增大而增加(P<0.01)。分别预孵育18β-GA和2-APB后,1×10-4 mol/L的丙泊酚对大鼠肠系膜动脉舒张作用明显减弱(P<0.01)。结论丙泊酚呈浓度依赖性舒张肠系膜动脉,其作用机制可能与缝隙连接通讯相关。

Objective To investigate the effects of propofol on mesenteric artery in SD rats and to observe whether the effect of propofol on the mesenteric artery relaxation is related to the gap.Methods Pressure myograph was used to examine the effect of 18β-GA and 2-APB on the relaxation induced by propofol 1×10-7,3×10-7,1×10-6,3×10-6,1×10-5,3×10-5,1×10-4 and 3×10-4mol/L in acutely separated mesenteric arterioles of SD rat.Results The diameter of mesenteric arterioles were increased from（208.6±13.4）to（213.5±13.6）,（219.7±13.2）,（226.4±12.5）,（234.9±12.3）,（245.5±13.0）,（267.4±15.2）,（336.2±18.3）and（385.9±14.2）μm after application of1×10-7,3×10-7,1×10-6,3×10-6,1×10-5,3×10-5,1×10-4 and 3×10-4 mol/L propofol,respectively.Propofol induced dilation of the rat mesenteric arterioles in a concentration-dependent manner（P〈0.01）.After pretreatment with 18β-GA and 2-APB,1×10-4 mol/L propofol induced dilation was absolutely decreased（P〈0.01）.Conclusion These results suggest that propofol relaxes mesenteric arterioles via gap junction.