P(GMA-co-MA)-g-PEO纳米纤维膜酶载体的制备与表征

Preparation and characterization of P(GMA-co-MA)-g-PEO nanofiber enzyme carrier

以聚乙二醇甲基丙烯酸甲酯(PEGMEMA)、甲基丙烯酸缩水甘油酯(GMA)和丙烯酸甲酯(MA)为原料,用偶氮二异丁腈(AIBN)引发合成P(GMA-co-MA)-g-PEO共聚物。通过静电纺丝制备了P(GMA-co-MA)-g-PEO纳米纤维膜酶载体,并用傅立叶红外光谱、热失重分析对载体进行结构表征。通过扫描电镜,探究了不同纺丝工艺下纳米纤维膜形态。通过静态接触角、动态接触角研究了纳米纤维膜酶载体的亲水性能。结果表明:在纺丝电压18kV、纺丝距离18cm、纺丝速度为0.1mL/h的工艺下,载体纤维直径为671nm,均匀度较好;载体具有一定的亲水性能。

P（GMA-co-MA）-g-PEO copolymer was prepared with glycidyl methacrylate（GMA）,ethyl acrylate（MA）and poly（etylene glycol）methyl ether methacrylate（PEGMEMA）as monomer and azodiisobutyronitrile（AIBN）as initiator.P（GMA-co-MA）-g-PEO nanofiber carrier was prepared by electrospinning,and the structure of carrier was characterized by fourier transform infrared spectroscopy（FT-IR）and thermogravimetric analysis（TGA）.By scanning electron microscopy,the morphology of nanofibers in different spinning processes was explored.The hydrophilic property of carrier was studied by static contact angle and dynamic contact angle.The results showed that the fiber morphology was better in the process which spinning voltage was 18 kV,the spinning distance was 18 cm,the spinning speed was 0.1mL/h,carrier fiber diameter was 671 nm,and the nanofiber carrier was hydrophilic.