大疱性类天疱疮患者外周血Th17及IL-17水平的检测

Th17 and IL-17 Levels in Peripheral Blood of Bullous Pemphigoid Patients

目的探讨Th17及其细胞因子IL-17在大疱性类天疱疮(bullous pemphigoid,BP)发病中的作用。方法实验组为27例BP患者,分别采集治疗前、后外周静脉血,对照组为27例健康体检者,收集外周静脉血。采用酶联免疫吸附试验(ELISA),检测BP患者治疗前、后及对照组血清中IL-17的浓度;实时荧光定量反转录聚合酶链反应(RT-PCR)技术检测外周血单个核细胞(PBMC)中IL-17 mRNA的表达水平;流式细胞术(FCM)检测外周血中Th17细胞占CD4+T细胞的百分比。结果 BP患者治疗前外周血中IL-17为32.34±9.55 ng/L,明显高于治疗后(17.47±3.01 ng/L)和对照组(14.74±2.77 ng/L),差异有统计学意义(P均<0.05);患者治疗前PBMC中IL-17 mRNA的表达量是治疗后的2.475倍(P<0.05),是对照组的2.980倍(P<0.05),治疗后与对照组比较,差异无统计学意义;BP患者治疗前外周血Th17细胞比例(CD4^+IL-17^+/CD4^+T细胞,3.280%±0.197%)显著高于治疗后(0.807%±0.034%)和对照组(0.675%±0.019%)(P<0.05)。而治疗后与对照组相比,差异无统计学意义。结论 Th17细胞及其细胞因子IL-17参与了BP的发病机制,并发挥着重要作用。

Objective To investigate the role of Th17 and IL-17 in the onset of bullous pemphigoid（BP）.Methods Peripheral bloods were obtained from 27 BP patients（before and after treatment respectively）and 27 normal controls. Enzyme linked immunosorbent assay（ELISA）was performed to detect the level of serum IL-17. Reverse transcription-polymerase chain reaction（RT-PCR）was applied in the quantitative analysis of the IL-17 mRNA expression in mononuclear cells in peripheral blood. Flow cytometry（FCM）was carried out to detect the proportion of Th17 cells. Results Elevated expression of IL-17 was noted in pre-treatment serum of BP patients（32.34±9.55 ng/L）compared with post-treatment serum（17.47±3.01 ng/L）and the serum of normal controls（14.74±2.77 ng/L）（both P〈0.05 ）. The level of IL-17 mRNA in pre-treatment stage of BP patients was 2.475 times as many as that in post-treatment stage, and was 2.980 times as many as that of normal controls（P〈0.05）. The proportion of peripheral blood Th17 cells in pre-treatment stage in BP patients（3.280%±0.197%）was higher than that in post-treatment stage（0.807%±0.034%）and in normal controls（0.675%±0.019%）（both P〈0.05）. Conclusion Th17 and the IL-17 are not only involved in the pathogenesis of BP but also play an important role in the development of disease.