摘要
利用RACE技术从艾草(Artemisia argyi)的成熟叶片中克隆了萜类化合物生物合成的单萜合成酶(TPS)的基因,并对其进行序列特征分析和系统进化关系预测。结果表明,该基因c DNA全长为1.7 kb,开放阅读框可编码538个氨基酸残基,并包含与其功能高度相关的DDxx D保守区和RR(degenerated RRx8W)保守区。预测的Aa TPS二级结构具有28个转角,30个α螺旋,8个β折叠。预测的三维结构符合单萜合酶的立体构象。系统发育分析表明,Aa TPS基因与其他双子叶植物中的单萜合酶基因聚为一类,与菊科植物的单萜合酶基因相似性较高。
To obtain the indispensable key enzyme involved in the monoterpene biosynthesis, the monoterpene synthase gene（TPS） was cloned and analyzed from Artemisia argyi through RACE. Results showed that the clones of c DNA were 1.7 kb on average, and contained an open reading frame（ORF） predicting a polypeptide of 538 amino acids（aa）, with a transit peptide and highly conserved DDxx D motif and RR（degenerated RRx8W） motif. Prediction of secondary structure and subcellular localization suggested that the protein might be encoded by Aa TPS containing28 coils, 30 alpha helixes, and 8 beta strands. The prediction of the three-dimensional structure conformed to spatial conformat of monoterpene synthase. Phylogenetic analysis indicated that Aa TPS grouped with other dicotyledonous plant mono-TPS, and subgrouped in the composite family.
出处
《热带作物学报》
CSCD
北大核心
2016年第7期1349-1356,共8页
Chinese Journal of Tropical Crops
基金
四川省科技厅科技支撑计划项目(No.2016NZ0058)
四川省教育厅重点科研项目(No.16ZA0323)
关键词
艾草
单萜合酶基因
系统发育分析
Artemisia argyi
Monoterpene synthase
Phylogenetic analysis
