西洋参蛋白酶解物对3T3细胞氧化损伤的保护作用研究

Protective effects of American ginseng protein hydrolysate on 3T3 cell oxidative injury

目的:优化西洋参蛋白酶解工艺,研究酶解产物对H_2O_2诱导3T3细胞氧化应激损伤的保护作用。方法:以相对分子质量分布为指标,通过正交试验确定西洋参蛋白酶解的最优工艺,采用H_2O_2诱导3T3细胞氧化损伤模型,MTT法检测细胞存活率,比色法检测SOD和LDH酶活力变化。结果:西洋参蛋白酶解的最优工艺为碱性蛋白酶与西洋参蛋白比(g/g)为1:50,酶解温度为55℃,酶解pH为9.5,酶解时间为6 h。酶解物能促进氧化损伤细胞增殖,提高SOD酶活力,降低LDH酶活力。结论:西洋参蛋白酶解物对成纤维细胞的氧化应激损伤有保护作用。

Objective： To investigate the optimal preparation technology of American ginseng protein and study the protective effects on 3T3 cell oxidative injury. Methods： The optimum enzymolysis technology was confirmed through the orthogonal test by comparing the relative molecular weight distribution of American ginseng protein which had been hydrolyzed. The cell proliferation, activity of SOD and LDH were selected to assay the protective effects of different concentrations of hydrolysate on on 3T3 cell oxidative injury. Results： The optimum hydrolysis conditions was obtained as follows： alkaline protease and substrate ratio（g/g） was 1：50, enzymolysis temperature was 55 ℃, enzymolysis p H was 9.5, enzymolysis time was 6 h. The hydrolysate could promote cell proliferation, increase activity of SOD and reduce activity of LDH. Conclusion： The American ginseng protein hydrolysate could protect 3T3 cell from oxidative injury.