促性腺激素释放激素激动剂对子宫内膜基质细胞蜕膜化的影响

The roles of gonadotropin-releasing hormone agonist(GnRH-α)in the decidualization of endometrial stromal cells(ESCs)

目的研究促性腺激素释放激素激动剂(GnRH-α)对子宫内膜基质细胞蜕膜化的影响。方法体外原代培养人子宫内膜细胞并构建蜕膜化模型,用不同浓度的GnRH-α处理细胞,Real-Time PCR方法检测标志子宫内膜基质细胞蜕膜化程度的分子催乳素(prolactin,PRL)和胰岛素样生长因子结合蛋白-1(IGFBP-1)mRNA的表达,ELISA法检测其在细胞上清液中的蛋白表达。结果 10^(-8)g/ml浓度,10^(-7)g/ml浓度和10^(-6)g/ml浓度的PRL mRNA表达均增加,10^(-7)g/ml浓度和10^(-6)g/ml IGFBP-1mRNA的表达增加(P<0.05,P<0.01);蜕膜化72h10^(-6)g/ml浓度基质细胞上清液中PRL蛋白增加,10^(-7)g/ml浓度上清液中IGFBP-1蛋白增加(P<0.05)。结论 GnRH-α促进了子宫内膜基质细胞蜕膜化PRL和IGFBP-1mRNA的表达和上清液中PRL和IGFBP-1蛋白的分泌。GnRH-α可以促进对人子宫内膜基质细胞的蜕膜化。

Objective To study the roles of gonadotropin-releasing hormone agonist （GnRH-α） in the decidualization of endometrial stromal eells（ESCs）. Methods Culture primary human endometrial stromal cells,and build the decidualed human endometrial stromal cells model in vitro, treated with different concentrations of GnRH-α, Using Real- Time PCR to detect the the expression PRL（prolactin） and IGFBP-1 （insulin-like growth factor binding proteinl） mRNA, which is the marker of decidualization, ELISA was also used to detect its expression in the cell supernatant Results In 10^-8g / ml concentration,10^-7g / ml concentration and 10^-6g /ml PRL mRNA expression increased,in 10^-7g / ml concentration and 10^-6g / ml IGFBP-1 mRNA expression increased（＊ P〈0.05,＊＊ P〈0.01）; after decidualized for 72 hours,in 10^-6g / ml concentration PRL protein increased in the cell supernatant,in 10^-7g / ml concentration IG- FBP-1 protein increased （ ＊ P〈0.05）. Conclusion GnRH-α increase the expression of PRL and IGFBP-1 mRNA in the the decidualization of ESCs. and also increase the PRL and IGFBP-1 protein expression in the cell supernatant. GnRH-α can promot the the decidualization of ESCs.