Allicin对DSS诱导的小鼠结肠炎的治疗作用及可能机制

Therapeutical effect of Allicin for colitis mice induced by DSS and its possible mechanisms

目的:明确Allicin对DSS诱导的小鼠结肠炎的治疗作用并进一步分析可能的机制。方法:将24只8周龄雄性BALB/c小鼠随机分为3组:Control组:阴性对照;DSS组:2.5%DSS诱导结肠炎;Allicin组:2.5%DSS诱导结肠炎+Allicin治疗[10 mg/(kg·d),共7 d]。结果:与DSS组比较,Allicin组疾病活动度评分及肠道炎症评分显著降低(P<0.05)。Allicin治疗组小鼠肠上皮细胞凋亡数目显著低于DSS组。Allicin治疗组小鼠肠黏膜炎症因子(IL-6、IL-1β及TNF-α)水平显著低于DSS组,差异均具有统计学意义(P<0.05)。与DSS组比较,Allicin治疗显著抑制了肠黏膜p-JAK2及p-STAT3的表达(P<0.05)。结论:Allicin对DSS小鼠肠炎具有治疗作用,可能的机制包括抗炎,保护肠上皮细胞及抑制JAK2/STAT3信号通路等。

Objective： To analyze the therapeutical effect of Allicin for colitis mice induced by DSS and its possible mechanisms. Methods： A total of 24 male mice were randomly divided into Control group,DSS group（ 2. 5% DSS,7 days） and Allicin group[DSS treatment and Allicin,10 mg/（ kg·d）,7 d]. Disease activity index,inflammatory score,TUNEL and Western bolt were performed to analyze the effect of Allicin on colitis induced by DSS. Results： With DSS group,the Allicin group had lower disease activity score and inflammatory score（ P〈0. 05）. Allicin group had a lower number of intestinal epithelial cell apoptosis than that of DSS group,the difference was statistically significant（ P〈0. 05）. Western bolt analysis shown that Allicin treatment significantly depressed the expression of p-JAK2 and p-STAT3 in intestinal mucosa when compared with these of DSS group（ P〈0. 05）. Conclusion： Conclusion Allicin has significantly therapeutic effect on mice colitis induced by DSS,the possible mechanisms including anti-inflammatory effects,protected of the intestinal epithelial cells and inhibition of the JAK2 / STAT3 signaling pathways.