HPLC-MS同时测定复方阿胶浆中的5种药效成分

Simultaneous Determination of Five Effective Components in Compound Ejiao Jiang by HPLC-MS

目的建立同时测定复方阿胶浆中绿原酸、党参炔苷、毛蕊花糖苷、人参皂苷Rg1、人参皂苷Re等药效成分的定量方法。方法采用TSQ Quantum Access Max三重四级杆质谱仪,电喷雾负离子模式(ESI-);Agilent Zorbax SB-C_(18)色谱柱(250 mm×4.6 mm,5μm);以水-0.02%甲酸为流动相A,以乙腈-0.02%甲酸为流动相B,梯度洗脱,柱温为30℃。结果绿原酸、党参炔苷、毛蕊花糖苷、人参皂苷Rg1、人参皂苷Re分别在2.05~51.68μg·m L^(-1)(r=0.9993)、2.33~58.80μg·m L^(-1)(r=0.9996)、1.13~28.99μg·m L^(-1)(r=0.9995)、1.23~30.63μg·m L^(-1)(r=0.9992)、2.25~56.45μg·m L^(-1)(r=0.9993)范围内线性关系良好;加样回收率均在99.14%~99.82%之间,RSD均小于5%。结论该方法可操作性强、稳定,结果准确。应用该方法可对复方阿胶浆进行全面准确的质量评价。

Objective To develop a HPLC-MS method for the determination of five effective components（chlorogenic acid,lobetyolin,acteoside,ginsenoside Rg1,ginsenoside Re） in Compound Ejiao Jiang. Methods The analysis was performed with TSQ Quantum Access Max triple quadrupole mass spectrometer by electrospray ionization（ESI-）mode and on Agilent Zorbax SB-C_（18）（250 mm×4.6 mm,5 μm） chromatographic column. The column temperature was 30 ℃,and mobile phase was 0.02 % formic acid-water（phase A）and acetonitrile -0.02 % formic acid（phase B）.Results Good linearity was shown in the ranges of 2.05-51.68 μg·m L^（-1）（r=0.9993）for chlorogenic acid,2.33-58.80μg·m L^（-1）（r=0.9996） for lobetyolin,1.13-28.99 μg·m L^（-1）（r=0.9995） for acteoside,1.23-30.63 μg·m L^（-1）（r=0.9992）for ginsenoside Rg1,2.25-56.45 μg·m L^（-1）（r=0.9993） for ginsenoside Re. The average recoveries of the five active components were in the range of 99.14 % ~ 99.82 % with RSDs being less than 5 %（n=6）. Conclusion The method is practical,accurate and stable,which can be used for the overall evaluation of the quality of Compound Ejiao Jiang.