摘要
应用绵羊的干扰素tau基因IFNt,根据毕赤酵母(Pichia pastoris)密码子选择偏好进行分子改造。改造后的基因IFNt克隆到毕赤酵母表达载体p PIC9上,构建得到重组毕赤酵母表达质粒p PIC9-IFNt。电转化于毕赤酵母GS115菌株,对转化后菌体诱导表达,上清SDS-PAGE检测,并优化诱导表达条件。结果表明:IFNt基因已重组到酵母染色体上,并能正常分泌表达,最佳诱导条件为:200 r/min,28℃,0.5%甲醇,96 h。该研究为真核表达绵羊IFNt的生产应用提供了一定的理论依据。
Ovine interferon tau（IFNt）gene was synthesized in terms of condon selection preference of Pichia pastoris and inserted into the vector p PIC9. The recombined p PIC9-IFNt was trnasformed into GS115 by electroporation and induced under optimized conditions,expression was detected by SDS-PAGE. The result showed that PIC9-IFNt was secretory expressed normally and optimized condition was 200 r/min,28℃,0.5%methanol,96 h. The study that supplied a relative productive evidence of IFNt eukaryotic expression.
出处
《中国草食动物科学》
CAS
2016年第4期5-7,10,共4页
China Herbivore Science
基金
北京自然基金(6132006)
