羔羊腹泻病原菌常规分离鉴定及药敏试验

Isolation,Identification of Lamb Diarrhea Pathogens and the Screening of Sensitive Drugs

从安微省合肥、滁州地区的肉羊养殖场中共采集到羔羊腹泻粪样11份,进行细菌分离培养与镜检,将分离的细菌进行纯培养,并进行生化鉴定、药敏试验和小鼠攻毒致病性检验。结果显示:在检测的11份粪样中,共检测出大肠杆菌11株、奇异变形杆菌7株;致病性试验显示大肠杆菌和奇异变形杆菌均对小鼠有致病力,但奇异变形杆菌致病性弱于大肠杆菌;药敏试验显示分离菌株对恩诺沙星、氟苯尼考、卡那霉素、丁胺卡那霉素、菌必治、复方新诺明、丙氟哌酸、头孢唑啉等敏感,对庆大霉素、氟哌酸等常规药物均表现出一定的耐药性,对青霉素、链霉素、克拉霉素、红霉素和强力霉素等完全耐药。结果表明,大肠杆菌和奇异变形杆菌都有一定的致病性,上述地区羔羊腹泻是由混合感染引起,且混合感染致病性更强。

In order to isolate and identify the pathogens using conventional methods as well as select the useful drug treatment for lamb diarrhea,the 11 fecal samples from lambs with diarrhea were collected from the mutton sheep/goat farms in Hefei and Chuzhou city. 11 samples of E. coli and 7 samples of Proteus mirabilis in the 11 fecal samples were detected,then the separated bacteria was purely cultured,biochemically identified,the drug sensitive test and the toxic pathogenic experiments in mice were carried out. The pathogenicity test showed that E. coli and Proteus mirabilis were virulent to mice,but the pathogenicity of Proteus mirabilis was weaker than that of the E. coli. The drug sensitivity test showed that enrofloxacin,florfenicol,kanamycin,amikacin,ceftriaxone,cotrimoxazole,norfloxacin propionate and cefazolin were effective to the seperated bacteria;gentamicin and norfloxacin a little effective,penicillin,streptomycin,clarithromycin and doxycycline ineffective. It is concluded that E. coli and Proteus mirabilis have certain pathogenicity,lamb diarrhea in these regions is caused by the mixed infection,and which is more pathogenic.