红花籽粕降糖有效部位的纯化工艺

Purification of antidiabtic compositions from safflower meal by macroporous resin

目的研究以大孔吸附树脂纯化的方法制备红花籽粕降糖有效部位的最佳工艺。方法以静态吸附法考查11种大孔树脂对有效部位的吸附和解吸附性能,以纯化后有效部位产率和酶活性数据进行综合评分,选出纯化效果最佳的树脂进行下一步动态筛选研究,并确定最佳纯化工艺。结果经过静态吸附-解吸附实验筛选出AB-8型大孔树脂为最佳纯化树脂;动态筛选结果得出最佳纯化工艺为:上样药液质量浓度为75 mg/m L;上样量为6 BV;吸附速率为3 BV/h。洗脱前先用水洗至还原糖阴性反应,再分别用10%、70%、95%乙醇以3 BV/h的速度洗脱,收集10%乙醇洗脱液6 BV和95%乙醇洗脱液5 BV,合并洗脱液,干燥后即获得有效部位。结论经AB-8型大孔树脂纯化处理后的红花籽粕有效部位降糖活性明显提高。此方法简便易行、成本低,可以用于工业化生产红花籽粕降糖有效部位。

Objective To explore the best way on purification hypoglycemic effective parts from safflower seed meal by using macroporous adsorption resin. Methods 11 kinds of maeroporous resin were screened by comparing the ability of adsorption and desorption performance for effective parts, the production and the inhibition on protein-tyrosine phos- phatase -1B and alpha glycosidase, which are two key enzymes in diabetic drug screening, were combined to choose the most suitable maeroporous resin for purifying the antidiabetic fraction; then the best one was subjected to dynamic absorption screening to optimize the best purification process. Results AB-8 macroporous resin was the best purifica- tion resin, and the optimization process was： concentration of sample was 75 mg/mL, sample volume on adsorption was 6 BV, flow rate on adsorption was 3 BV/h, elution by water to reduce the sugar negative reaction, flow rate of adsorp- tion and elution was 3 BV/h for 4 BV, then elution solvent was 10%, 70%, 95% ethanol, and 6 BV 10% and 5 BV 95% ethanol part were collected. Conclusion Compared with the extraction before purification, purified parts sig- nificantly inhibit PTP-1B and alpha glycosidase. This method is simple and low cost, it can be used for pro- ducing antidaibetic parts from safflower seed meal in in- dustry.