苜蓿根瘤菌的发酵工艺优化

Optimization of the fermentation conditions of Alfalfa Rhizobia

试验对1株苜蓿根瘤菌M2进行了培养条件及发酵配方的优化。通过设置不同的温度、pH值、转速,并调整发酵配方,得到最适发酵工艺：最适温度为30℃,最适pH值为灭菌前7.5,最适转速为180 r/min。经进一步调整配方后,菌数可稳定在90×10~8 cfu/m L。

This research carried out the culture condition s and fermentation formula optimization of alfalfa rhizobia M2.By setting the different temperature, pH, speed, and adjusting the fermentation formula, we got the optimal fermentation process.The optimal temperature was 30 ℃, the optimum pH was 7.5 before sterilization, the optimal rotate speed was 180 r/min.After further formula adjustment,the bacterium number was stable at 90×10~8cfu/m L.