不同硫熏替代技术的黄芪药材对小鼠免疫功能影响研究

Effect of Astragali Radix with different sulfur fumigation technologies on immune function in mice

研究不同硫熏替代技术的黄芪药材对小鼠免疫功能的影响。观察不同黄芪样品对环磷酰胺诱导免疫低下小鼠碳廓清的影响、对免疫低下小鼠免疫器官质量的影响以及对2,4-二硝基氯苯所致小鼠迟发型超敏反应(DTH)的影响。以碳廓清指数、吞噬指数、脏器指数、耳肿胀度为指标,结果显示,不同硫熏替代技术的黄芪药材均能显著提高免疫低下小鼠的碳廓清指数K和吞噬指数α,明显增强免疫低下小鼠的免疫器官质量和提高DTH反应能力,其中,与模型组相比,热风微波组均有最显著差异,但各组与热风微波组比较,只有在碳廓清实验中有显著性差异。从药效学角度,考察了不同硫熏替代技术的黄芪药材对小鼠免疫功能的影响,为优选合适的替代技术提供参考依据,也为临床用药提供了指导性意见。

To investigate the effect of Astragali Radix with different sulfur fumigation technologies on immune function in mice, and observe the effect of different Astragali Radix samples on carbon clearance in cyclophosphamide induced immunosuppressed mice, on immune organ weight in immunosuppressed mice and on delayed type hypersensitivity response （DTH） induced by 2,4 dinitrochlorobenzene. Carbon clearance index, phagocytic index, organ index and ear swelling rate were taken as the indexes. The results showed that, all of the Astragali Radix with different sulfur fumigation technologies markedly increased the carbon clearance index K, phagocytic index a, immune organ weight and improved the ability of DTH response in immunosuppressed mice. As compared with the model group, combined hot air-microwave group had the most significant difference, but when other groups were compared with and combined hot air-microwave group, only carbon clearance test had significant difference. From the perspective of pharmacodynamics, the effect of Astragali Radix with different sulfur fumigationon technologies on the immune function of mice was investigated, which provided a reference for the selection of appropriate alternative technology, and also pro- vided guidance for clinical medication.