血必净对热休克蛋白90在脓毒症小鼠肝肺损伤中表达的影响

Effect of Xuebijing on the expressions of HSP 90 in liver and lung injury of the septic mice

目的：检测脓毒症小鼠热休克蛋白90（HSP90）水平的变化及血必净注射液干预对脓毒症的作用。方法：84只小鼠随机分成脓毒症模型组、血必净干预组,制模后0 h、1 h、2 h、4 h、8 h、12 h和18 h,测定血清谷氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、HSP90含量,肺和肝组织HSP90表达量,HE染色观察肝、肺组织的病理变化。结果：干预组小鼠血清AST水平在给药后2 h、4 h和8 h均高于模型组（P〈0.05~P〈0.01）,而血清ALT水平在给药后1 h、4 h和8 h均高于模型组（P〈0.05~P〈0.01）。模型组小鼠血清、肝和肺组织中HSP90在给药后2~18 h均显著高于干预组（P〈0.01）,病理切片观察肝、肺组织,模型组中炎症逐渐加重,肺泡壁被炎症破坏,肺泡间隔增宽,间质纤维组织增生,大部分肝细胞出现了气球样变性,小叶点状坏死,肝汇管区出现了桥接坏死;而干预组炎症显著降低。结论：脓毒症时,HSP90表达上调,提示其参与了免疫应答和免疫调节反应。血必净注射液在脓毒症时可降低血清及肝、肺组织中的HSP90,减轻脓毒症的炎症反应及肝脏、肺脏病理损伤。

Objective： To detect the expression of heat shock protein 90（ HSP90） in septic mice,and investigate the effects of Xuebijing on sepsis. Methods： Eighty-four mice were randomly divided into the sepsis model group（ model group） and Xuebijing inhibition group（ inhibition group）. The serum levels of ALT,AST and HSP90,and HSP90 expressions and pathological changes in lung and liver tissues were detected after 0 h,1 h,2 h,4 h,8 h,12 h and 18 h of molding. Results： The serum levels of AST in inhibition group were higher than that in model group after 2 h,4 h and 8 h of administration（ P〈0. 05 to P〈0. 01）,but the serum levels of ALT in inhibition group were higher than that in model group after 1 h,4 h and 8 h of administration（ P〈0. 05 to P〈0. 01）. The HSP90 levels in serum,and liver and lung tissues in model group were significantly higher than that in inhibition group after 2 h to 18 h of administration（ P〈0. 01）. Histopathology showed that the inflammation of lung and liver tissues gradually aggravated,the alveolar wall destructed,the alveolar septum widened,the interstitium proliferated,part of liver cells ballooning degenerated,spotty Leaflet necrosed,and hepatic portal bridging necrosis occurred in model group. The inflammation reaction in inhibition group significantly decreased.Conclusions： The expression of HSP90 in sepsis mice upregulates,which indicates that the HSP90 involves in the immune response and regulation. The Xuebijing can decrease the levels of HSP90 in liver and lung tissues,alleviate the inflammation reaction and pathological lesion of liver and lung tissues in sepsis mice.