巢蛋白在人胚胎消化管的表达

The expression of nestin in human fetal digestive tract

目的通过研究不同时期人胚胎消化管巢蛋白的表达及其变化探讨巢蛋白在消化管发育过程中的作用。方法取3-8月人胚胎食管、胃和小肠,常规石蜡切片,采用免疫组织化学法检测巢蛋白在胚胎消化管中的表达及分布。结果各胎龄段（3-8月）消化管均有巢蛋白的表达。巢蛋白在消化管主要表达在固有层、黏膜下层和肌层内的微血管和毛细血管壁上,且巢蛋白的表达随胎龄的增加而减少。与发育早期组相比,发育中期、晚期胚胎食管、胃和小肠组织中巢蛋白的表达显著减少（P〈0.05）,差异有统计学意义。而在发育中期和发育晚期巢蛋白的表达无显著性差异（P〉0.05）。结论巢蛋白在胎儿消化管壁的微血管和毛细血管的管壁上表达,且随着胎儿发育,其表达逐渐减少,提示巢蛋白在这些器官组织的血管形成过程中起作用。

Objective To investigate the function of nestin during digestive tract development by analyzing expression of nestin in human fetal digestive tract. Methods Human fetal esophagus, stomach,and small intestine of 3- 8 months old were obtained and fixed for paraffin sections. Immunohistochemical staining was used to determine the expression and distribution of nestin in fetal esophagus, stomach and small intestine. Results Nestin was expressed in the blood capillaries of lamina propria, submucosa and muscularis layers in digestive tract. The expression of nestin in these tissues was decreased with the development of fetus. There was statistical difference between nestin expression in early development stage and medium or late stage（P〈0.05）, but no statistical difference between medium and late development stages（P〉0.05）. Conclusion Nestin is expressed in capillary of different tissues; however, the expression levels are decreased with the fetal development which indicates that nestin might play a role in angiogenesis.