广西肝癌高发区乙型肝炎病毒基因型分析

Analysis of hepatitis B virus genotype in regions in Guangxi with high incidence of primary liver cancer

目的:探讨广西肝癌高发区乙型肝炎病毒(HBV)基因型的分布特征。方法:按性别及年龄配对,收集肝癌高发家族(试验组)和无癌家族(对照组)中的成员各103例,入选者均为HBV感染者。采用型特异性引物巢式PCR及测序的方法进行基因型的检测。结果:研究对象共检出B、C、B/C混合、D 4种基因型,它们在两组中的比例分别为31.1%、63.1%、1.9%、1.9%和30.1%、55.3%、6.8%、2.9%,差异均无显著性(P>0.05)。HBeAg阳性率C基因型高于B基因型(P<0.05),B、C基因型在性别、年龄、民族、HBV DNA水平上差异均无统计学意义(P>0.05)。结论:广西肝癌高发区HBV以B、C基因型为主,有少量的B/C混合型和D基因型。广西肝癌高发与基因型关系不大。

Objective To explore the distribution and characteristics of hepatitis B virus（HBV） genotype in the region in Guangxi with high incidence of primary liver cancer（PLC）. Methods 103 pairs of samples from the sex- and age-matched members with HBs Ag-positive from PLC-clustering families（the experimental group）and carcinoma-free families（control group） were collected. Nested polymerase chain reaction（PCR） and sequencing methods were applied for the analysis of HBV genotype. Results Four HBV genotypes： B, C, B / C and D,were detected, the percentages of them in the two groups were 31.1%, 63.1%, 1.9%, 1.9% and 30.1%, 55.3%,6.8%, 2.9%, respectively, showed no significant differences（P〈 0.05）. HBe Ag positive rates were significantly different between genotype C and B（P〈 0.05）, but no significant differences were showed in such factors as gender, age, ethnic group and HBV DNA level in them（P〈 0.05）. Conclusions The main genotypes were types B and C besides a small number of combined genotypes B / C and D in the regions of Guangxi with a high incidence of PLC. There may be few relationships between HBV genotypes and the high incidence of PLC for familial clustering in Guangxi.