小球藻抽脂残留物培养产气肠杆菌的制氢研究

Cultivation of Enterobacter Aerogenes for Hydrogen Production with Lipid Extracted Microalgal Biomass Residues

为使高效产氢产气肠杆菌能够运用到实际生产中,探索并且优化以小球藻抽脂残留物的水解产物为底物的厌氧批次产氢发酵实验参数.采用中心组合设计,通过三次平行实验取得的数值,拟合得到反映温度、接种量和p H值与产氢量之间关系的多元二次回归模型,以产氢率为响应值,进行响应面分析.方差分析结果显示,该模型的显著性和可靠性较高,拟合效果良好.该模型预测出最佳产氢结果为54.22 m L/g小球藻抽脂残留物,产氢条件为温度37.55℃,接种量12.25%,p H值5.95.进行了厌氧批次发酵产氢验证实验,实际结果为小球藻抽脂残留物的产氢量为54.61 m L/g,与预测值十分接近,说明该模型能较好反应三因素对产氢量的影响.优化了高效产氢菌利用廉价底物发酵产氢的运行条件,为实现生产氢气的过程与有机废弃物无害化处理相耦合提供了新思路.

To examine the feasibility of cultivating Enterobacter aerogenes for hydrogen production with lipidextracted microalgal biomass residues,the anaerobic batch fermentations from microalgal hydrolysate wereconducted and their key parameters were optimized using response surface methodology.The central compositedesigns were performed,and a quadratic regression model based on temperature,p H,inoculum and hydrogenyield was obtained from the triplicate experimental data.The analysis of variances indicates that the model hasgood fitting degree.The predicted maximum hydrogen yield of 54.22 m L/g of lipid extracted microalgal bio-mass residues was obtained when the temperature,p H and inoculum were respectively at 37.55 ℃,5.95 and12.25%.The confirmatory experiments showed that the mutant evolved hydrogen yield of 54.61 m L/g of lipidextracted microalgal biomass residues in the optimal conditions.The coincident result verified the practicabilityof the model.This study indicates that a strategy of cultivating Enterobacter aerogenes for hydrogen productionwith lipid extracted microalgal biomass residues has great potential for the large scale of production.