奶牛子宫内膜炎病原菌分离与药敏试验

Identification and Drug Sensitivity Test of the Pathogenic Microbe of Dairy Cattle Endometritis

本试验对采自山西省某牛场30头奶牛子宫内膜炎患牛的病料进行了病原菌分离与药敏试验,主要分离出3种疑似致病菌,经生化试验鉴定,其中葡萄球菌14株,阳性率为46.7%(14/30);链球菌11株,阳性率为36.7%(11/30);大肠杆菌5株,阳性率为16.7%(5/30)。药敏试验结果表明,恩诺沙星、卡拉霉素、诺氟沙星的抑菌效果明显,可作为奶牛场治疗子宫内膜炎的首选药物;链霉素、青霉素的抑菌效果相对较差。建议用药时需合理配伍,不可随意加大剂量,同时几种药物要交替使用,以免产生耐药菌株,影响治疗效果。

Pathogens were separated from 30 dairy cattles which suffered from endometritis and were analyzed by medicine sensitive test. The results showed that the uterine secretion derived pathogens included three major kinds of bacterias： 14 strains of Staphylococcus aureus （46.7%）, 11 strains of Streptococcus （36.7%）, 5 strains of Escherichia coli （16.7%）. The medicine sensitive test showed that, enrolfoxacin, kalamycin, norlfoxacin may observably inhibit the growth and proliferation of pathogens and may be the primary candidate for endometritis therapy in dairy cattle farm. However, the pathogens were insensitive to streptomycin and penicillin. As a conclusion, to avoid drug-resistant and maximize the therapeutic efficacy, compatibility of medicines should be applied in a reasonable ratio and the dosage should be controlled and not be increased casually. Meanwhile, different kinds of medicine should be used alternately.