我国中东部地区蜱中斑点热群立克次体与新型布尼亚病毒复合感染调查研究

CO-INFECTION OF SPOTTED FEVER GROUP RICKETTSIAE AND SEVERE FEVER WITH THROMBOCYTOPENIA SYNDROME VIRUS IN TICKS IN EASTERN CENTRAL CHINA

为了验证我国中东部发热伴血小板减少综合征流行地区-信阳和济南的蜱中有斑点热群立克次体存在，且有复合感染新型布尼亚病毒的情况，用布旗法和动物体表检查法在河南省信阳市和山东省济南市捕获蜱，采用PCR和RT-RCR进行病原体感染筛查。信阳市捕获长角血蜱215只，其中游离蜱164只，寄生蜱51只。37只蜱斑点热群立克次体检测阳性，阳性率17．2％；38只蜱新型布尼亚病毒检测阳性，阳性率为17．7％；4只蜱斑点热群立克次体与新型布尼亚病毒复合感染，复合感染率为1．9％。山东省捕捉到166只寄生蜱，均为长角血蜱，4只蜱斑点热群立克次体检测阳性，阳性率为2．4％。6只蜱新型布尼亚病毒检测阳性，阳性率为3．6％；2只蜱为斑点热立克次体与新型布尼亚病毒复合感染，复合感染率为1．2％。结果证实我国中东部地区存在斑点热立克次体与新型布尼亚病毒复合感染。

The aim of present study is to investigate the co-infection of spotted fever group rickettsiae （SFGR） and severe fever with thrombocytopenia syndrome virus （SFTSV） in eastern central China. Ticks were collected by flag-drag method and checking ectoparasites from animal body surface, followed by detection of pathogens with PCR and RT-PCR. A total of 215 adult Haemaphysalis longicornis ticks were collected from Xinyang City （Henan Province） including 164 host-seeking ticks and 51 parasitic ticks. Of the 215 ticks 37 （ 17. 2% ） were tested positive for SFGR, 38 （ 17. 7% ） were positive for SFTSV, and 4 （ 1. 9% ） were co-infected with SFGR and SFTSV. A total of 166 adult Haemaphysalis longicornis ticks, all were host-seeking ones, were collected from Jinan city （Shandong province）. Of the 166 ticks 4 （2. 4% ） were tested positive for SFGR, 6 （3. 6% ） were tested positive for SFTSV, and 2 （1. 2% ） were co-infected with SFGR and SFTSV. The results of present study reveal that the co - infection of SFGR and SFTSV is real existed in ticks distributed in eastern central China.