37℃生理盐水滴注后大鼠鼻黏膜病理改变观察

Pathological changes of nasal mucosa after instillation of 37℃ normal saline in rats

目的观察37℃生理盐水滴注后大鼠鼻黏膜病理学的改变。方法48只大鼠以37℃生理盐水滴入双鼻中隔黏膜30次,每分钟1次。另设2只为对照。于滴鼻后第15、30分钟及第1、3、7、14、28、42天各取6只大鼠相同刺激区鼻中隔黏膜行光镜、电镜观察,对照组取相应区黏膜观察。结果光镜下1、3、7天见细胞排列紊乱,血管腺体结构无损伤,14天后恢复。电镜下第30分钟、第1、3天见细胞水肿、核周隙扩张,纤毛、微绒毛紊乱短少,7天后开始恢复,28天完全恢复。结论37℃生理盐水滴入鼻腔对大鼠的鼻黏膜上皮可造成损伤,损伤程度轻,表现为纤毛微绒毛的紊乱短少和上皮细胞水肿,恢复快。损伤于滴入后30分钟开始发生,3天内达高峰,7天后开始恢复,28天完全恢复。

[ABSTRACT]OBJECTIVE To obser ve t he pathological changes of nasal mucosa in rats after instillation of 37℃ normal saline. METHODS Forty-eight qualified＆amp;nbsp;rats were given instillation of 37℃ nomal saline 30 times, once per minute. Another 2 qualified rats served as control. After instillation, in the 15, 30 minutes and 1st, 3rd, 7th, 14th, 42th, 28th day respectively, the nasal septum mucosa of 6 rats was observed by light and electron microscopy. The corresponding area of the mucosa of control group was also observed by light and electron microscope. RESULTS Under light microscope, the arrangement of cell was disordered in the 1st, 3rd, 7th days. But there is no damage of vascular gland structure. After 14 days, the disorder recovered. With electron microscope, edema of cells, expansion of perinuclear gap, disorder of cilia and microwilli was found in the 30 minutes, 1st, 3rd day. All these began recover in the 7th days, and completely restored in the 28th day. CONCLUSION 37℃ nomal saline drip can damage nasal mucosa, but the damage is light, which is characterized by disorder and shortage of cilia and microvilli, edema of the epithelial cells. And the damage can recover quickly. It began in the 30 minutes after instillation, reached its peak in the 3rd day. And the recovery began in the 7th day, and completed in the 28 day.