HPLC-ELSD法同时测定小儿宝泰康颗粒中贝母素甲与贝母素乙的含量

Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD

目的建立HPLC-ELSD同时测定小儿宝泰康颗粒中2个指标成分贝母素甲、贝母素乙含量的方法。方法采用Thermo C18（250mm×4.6mm,5μm）色谱柱,流动相：乙腈-水-二乙胺（65∶35∶0.03）,流速：1.0ml/min;ELSD参数为漂移管温度：85℃,载气流速：2.2L/min。结果贝母素甲在0.586~5.860μg（r=0.999 6）、贝母素乙在0.564~5.640μg（r=0.999 9）与峰面积的对数值呈线性关系;平均加样回收率分别为99.44%和98.56%,RSD分别为0.7%和1.0%。结论该方法简便、准确、重复性好,可用于小儿宝泰康颗粒的质量控制。

Objective To develop a new high performance liquid chromatography（HPLC）coupled with Evaporative Light Scattering Detector（ELSD）method for simultaneous determination of two indicative components（peimine and peiminine）in Xiaoer Baotaikang granules.Methods The analysis was performed on a Thermo C18column（250 mm × 4.6 mm,5μm）by using a mobile phase consisted of acetonitrile-water and diethylamine（65∶35∶0.03）,and the flow rate was1.0ml/min;ELSD parameters：the temperature of drift tube was 85℃and the carrier gas flow rate was 2.2L/min.ResultsThe linear ranges of peimine and peiminine were 0.586~5.860μg（r=0.999 6）and 0.564~5.640μg（r=0.999 9）,respectively.The average recovery rate for peimine and peiminine was 99.44%（RSD was 0.7%）and 98.56%（RSD was 1.0%）,respectively.Conclusions The method is sample,accurate and reproducible,which could be used for the quality control of Xiaoer Baotaikang granules.