摘要
为开发防治黄瓜枯萎病的生物制剂奠定基础,通过对已筛选的黄瓜枯萎病拮抗菌HM-7进行培养特征观察、显微观察、革兰氏染色、16S r DNA全序列分析、系统发育分析等研究,初步鉴定拮抗菌HM-7为解淀粉芽孢杆菌。芽孢杆菌属是研究较为广泛和深入的微生物类群之一,也是植物病害生物防治生防菌的主要来源之一,因此拮抗菌HM-7有望开发为生物制剂应用于农业生产。
It was studied that the initially identification of the selected antagonistic bacteria HM-7 of cucumber fusarium wilt in order to lay the foundation to the development of biological pesticide. HM-7 was identified according to the cultural characteristics in different medium and its individual form under a microscope, with 16S rDNA complete sequence analysis by comparing the consequences with standard strains and then construct a phylogenetic tree. The results showed that HM-7 was initially identified as Bacillus amyloquefaciens. Bacillus genus is one of microbial groups which were researched exten- sively and deeply, and it was one of the main sources of bio-control bacteria which applied in biological control of plant dis- ease. So HM-7 is expected to be developed for biological pesticide.
出处
《中国瓜菜》
CAS
北大核心
2016年第8期13-16,共4页
China Cucurbits And Vegetables
基金
河北省高等学校科学技术研究青年基金项目(QN2014323)
关键词
黄瓜
枯萎病
拮抗菌
鉴定
Cucumber
Fusarium wilt
Antagonistic bacteria
Identification
