摘要
将已构建的与减数分裂相关的OSD1基因的RNAi载体通过农杆菌转化香石竹品种‘Nogalte’,通过正交试验,筛选得出如下最佳转化条件:预培养4 d、黑暗下共培养5 d、农杆菌菌液浓度OD600=0.5、共培养基中AS浓度30 mg/L、农杆菌侵染时间30 min。
The RNAi vector of OSD1 gene which was connected with meiosis was constructed and integrated into carnation cultivar ‘Nogalte＇by Agrobacterium-mediated genetic transformation. Through orthogonal test,the optimal transformation conditions were screened out as follows： pre-culture for 4 days,co-cultivation in dark for 5 days,concentration（ OD600） of Agrobacterium suspension solution being 0. 5,concentration of AS（ acetosyringone） in co- cultivation medium being 30 mg / L,and infection time of Agrobacterium being 30 min.
出处
《江西农业学报》
CAS
2016年第7期27-31,共5页
Acta Agriculturae Jiangxi
基金
国家自然科学基金项目(31460530
31260491)
国家观赏园艺工程技术研究中心项目(2012FU125X10)
云南省应用基础研究计划(2014FA044)
云南省中青年学术和技术带头人培养计划(2015HB077)
关键词
基因工程
农杆菌介导
正交试验
香石竹
Genetic engineering
Agrobacterium-mediated transformation
Orthogonal test
Carnation
