基于代谢组学技术研究黄芩对小鼠代谢的影响

Effect of Scutellariae Radix on Metabolism in Mice by Metabolomics

目的:基于代谢组学技术研究黄芩对正常小鼠血清和肝脏内源性代谢产物的影响。方法:采用基于核磁共振(1H-NMR)代谢组学技术结合多元统计分析及单变量分析手段,比较正常小鼠与黄芩干预小鼠的血清和肝脏内源性代谢物的变化。结果:给予黄芩后小鼠机体内源性代谢物发生了明显变化。与空白组相比,黄芩组小鼠血清中的10种代谢物含量发生变化,如3-羟基丁酸、柠檬酸、胆碱等含量升高,α-葡萄糖和β-葡萄糖含量下降;肝脏中19种代谢物含量发生变化,如谷氨酸、异亮氨酸、亮氨酸、肝糖原、腺苷等含量升高,黄嘌呤等含量降低,这些内源性代谢物的改变涉及机体的糖代谢、脂肪代谢、氨基酸代谢等途径。结论:黄芩对正常小鼠机体代谢的影响不仅与其寒性有关,而且可能与其抗氧化、保肝、降糖等药理作用密切相关,可为黄芩的药性和药理作用机制的深入研究提供参考。

Objective： To investigate effect of Scutellariae Radix on endogenous metabolites in the serum and liver of normal mice based on metabolomics. Method：1H-NMR based on metabolomics,coupled with multivariate and univariate statistical analysis, was used to compare differential endogenous metabolites in the serum and liver between the normal mice and those treated by Scutellariae Radix. Result： Endogenous metabolism of mice treated by Scutellariae Radix had significant changes. Compared with the blank group,contents of 10 kinds of metabolites in serum had changed,contents of 3-hydroxybutyric acid,citric acid and choline increased,levels of α-glucose and β-glucose decreased in mice serum of Scutellariae Radix group; 19 kinds of metabolites in the mice liver of Scutellariae Radix group also changed,levels of glutamic acid,isoleucine and leucine increased,levels of xanthine decreased. Changes of these metabolites in mice of Scutellariae Radix group involved in glycometabolism,lipid metabolism and amino acid metabolism. Conclusion： Impact of Scutellariae Radix on metabolism of mice is related with the cold property of Scutellariae Radix,its anti-oxidative,hepatoprotective and hypoglycemic effect and other pharmacological action. This study can provide a reference for the further research of property and pharmacological mechanism of Scutellariae Radix.