复方土茯苓颗粒对HUA痰湿体质大鼠模型相关理化指标及蛋白水平的影响

Effect of Compound Tufuling Granules on Physical and Chemical Indexes and Protein Levels in Hyperuricemia Rats with Syndrome of Phlegm-Dampness

目的:观察复方土茯苓颗粒对高尿酸血症(HUA)痰湿体质大鼠模型的相关理化指标及蛋白水平的影响,并探讨其相关的作用机制。方法:48只大鼠随机分为正常组,模型组,西药组(非布司他,4 mg·kg-1),复方土茯苓颗粒低、中、高剂量组(1,2,4 g·kg-1),除正常组外,其余各组用高脂饲料联合次黄嘌呤ig,氧嗪酸钾ip制备HUA痰湿体质大鼠模型,并给予相应药物干预;30 d后测定各组大鼠行为学变化及血清中尿酸(UA),丙氨酸氨基转移酶(ALT),天门冬氨酸氨基转移酶(AST),甘油三脂(TG),低密度脂蛋白(LDL)和肾脏尿酸盐重吸收转运体1(m URAT1)蛋白的水平及肝脏病理变化。结果:模型组大鼠血清中UA,ALT,AST,TG,LDL和m URAT1蛋白的水平均较正常组显著升高(P<0.05),同时,复方土茯苓颗粒高剂量组大鼠血清中的UA,ALT,AST,TG,LDL和m URAT1蛋白的水平均比模型组要显著降低(P<0.05),并且痰湿证评分明显改善(P<0.05),复方土茯苓颗粒中剂量组大鼠血清中仅AST,TG,LDL水平与模型组比差异不大,余结果同复方土茯苓颗粒高剂量组,而复方土茯苓颗粒低剂量组与模型组无明显差别;而非布司他组仅血清UA水平与模型组比较显著降低(P<0.05),其中血清UA水平与复方土茯苓颗粒高剂量组比较无明显差异;模型组肝脏病理显示一定的肝损害。结论:复方土茯苓颗粒能降低高尿酸血症痰湿证血清UA水平,与非布司他比较更能改善痰湿型体质、肝功能和血脂,其机制可能与抑制m URAT1蛋白表达水平有关。

Objective： To observe the effect of compound Tufuling granules（ CTG） on physical and chemical indexes and protein levels in hyperuricemia（ HUA） rats with syndrome of phlegm-dampness. Method：Forty-eight rats were randomly divided into blank group,model group,western medicine group（ febuxostat,4mg·kg- 1）,CTG low-dose group,CTG mid-dose group and CTG high-dose group（ 1,2,4 g·kg- 1）. The rats in all groups except blank group received high-fat diets combined with hypoxanthine by intragastric administration and potassium oxygen by intraperitoneal injecting to establish hyperuricemia（ HUA） rat models with syndrome of phlegm-dampness,and then the rats were given with appropriate treatment. Thirty days later,the changes of behavioristics and serum uric acid（ UA）,alanine aminotransferase（ ALT）,aspartate aminotransferase（ AST）,triglyceride（ TG）, low density lipoprotein（ LDL）, protein levels of renal urate reabsorption transporter 1（ m URAT1） and liver pathology of all the rats were detected. Result： In model group,the levels of UA,ALT,AST,TG,LDL and m URAT1 protein levels were significantly higher than those in blank group（ P 〈0. 05）. In CTG high-dose group,the levels of UA,ALT,AST,TG,LDL and m URAT1 protein levels were significantly lower than those in model group（ P 〈0. 05）,and the scores of syndrome of phlegm-dampness were significantly improved（ P 〈0. 05）. The difference was not significant in levels of AST,TG,and LDL between CTG mid-dose group and model group,but the effects on other levels in mid-dose group were similar with those in CTG high-dose group.There was no significant difference between CTG low-dose group and model group. In western medicine group,only the level of UA was significantly lower than that of model group（ P 〈0. 05）,and there was no significant difference in the level of UA between western medicine group and CTG high-dose group. Liver damages were observed in liver pathology of model group. Conclusion： CTG may reduce the level of serum uric UA in HUA rats with phlegmdampness,improve the constitution of phlegm-dampness,liver function and blood lipids as compared with the western medicine,and the mechanism may be associated with down-regulating the protein levels of renal m URAT1.