非水毛细管电泳法分离14种氨基醇类手性药物

Chiral separation of fourteen amino alcohols by nonaqueous capillary electrophoresis

建立D-(+)-葡萄糖酸δ-内酯–硼酸络合酸作为手性选择剂分离14种氨基醇类手性药物的非水毛细管电泳(NACE)新方法。实验考察了D-(+)-葡萄糖酸δ-内酯浓度、硼酸浓度、三乙胺浓度以及毛细管温度对14种氨基醇类手性药物分离效果的影响。确定了最佳手性分离条件为:未涂层熔融石英毛细管柱(55 cm×50μm ID,有效长度45 cm);背景缓冲液组成为200 mmol·L^(-1) D-(+)-葡萄糖酸δ-内酯、80 mmol·L^(-1)硼酸、57.4 mmol·L^(-1)三乙胺的甲醇溶液;进样压力2.9 psi,时间2 s;毛细管温度25±0.2℃;运行电压+15 k V;检测波长214 nm。在最佳分离条件下,大部分手性药物获得了良好的分离。本文为多羟基化合物–硼酸络合酸新型手性选择剂的原位合成及其在非水毛细管电泳中分离手性药物的应用奠定了基础。

We developed a new method for chiral separation of fourteen amino alcohols by nonaqueous capillary electrophoresis（NACE） with the D-（＋）-gluconic acid δ-lactone-boric acid complex as chiral selector. In order to achieve good enantioseparation, the effects of D-（＋）-gluconic acid δ-lactone and boric acid concentrations, triethylamine concentration, as well as capillary temperature were systematically investigated. The optimized conditions were identified as follows： an uncoated fused silica capillary of 50 μm ID with a total length（L_（tot）） of 55 cm and an effective length（L_（eff）） of 45 cm; 200 mmol·L（-1） D-（＋）-gluconic acid δ-lactone, 80 mmol·L（-1） boric acid, and 57.4 mmol·L（-1） triethylamine in methanol; positive pressure injection at 2.9 psi for 2 s; capillary temperature, 25 ± 0.2 ℃; applied voltage, ＋15 k V; detection wavelength, 214 nm. Under the optimized conditions, a good chiral resolution was achieved in most of the tested drugs. This method provides a foundation for the development and application of new chiral selectors of polyhydroxy compound-boric acid complexes in chiral drugs analysis by NACE.