通脑疏脉丸毒理学及药效学实验研究

Experimental Study on Toxicology and Pharmacology of TongNao ShuMai Pills

目的:观察通脑疏脉丸的急性毒性反应、对血瘀证模型大鼠血液流变学的影响、对大鼠血小板聚集和解聚的影响。方法:对通脑疏脉丸进行小鼠急性毒性实验,因未测出小鼠致死剂量,无法测定LD50,故改测最大给药量;药效学实验采用通脑疏脉丸高剂量组、低剂量组分别灌胃通脑疏脉丸7.5 g/kg(体质量)、2.5 g/kg(体质量)。结果:小鼠灌胃给药通脑疏脉丸,未发现明显毒性反应,1日最大给药量为67.9g/kg(体质量),是临床人日用量的136倍;通脑疏脉丸高、低剂量可使经肾上腺素和冰水处理后造成的血瘀证模型大鼠全血黏度及血浆黏度明显降低(P<0.05),与模型对照组比较,差异有统计学意义(P<0.05)。通脑疏脉丸高、低剂量对ADP诱导大鼠全血血小板聚集有明显抑制作用,能明显降低1、6分钟电阻值,与模型对照组比较,差异有统计学意义(P<0.05)。结论:通脑疏脉丸1日内剂量过大口服是安全的,能明显降低大鼠全血及血浆黏度,抑制血小板聚集和促进血小板解聚,有改善血液流变学异常的作用。

Objective： To observe acute toxic reaction of Tong Nao Shu Mai pill and its effects on the hemorheology, platelet aggregation and depolymerization of the rat model of blood-stasis pattern. Methods： Acute toxic test of Tong Nao Shu Mai pill was conducted on the mice, because the lethal dosage and LD50 were not detected out, maximum administration dosage was tested. In the pharmacodynamic study, the rats were divided into low dosage group and high dosage group. They accepted Tong Nao Shu Mai pill in the concentrations of 7.5g/kg body mass and 2.5g/kg body mass respectively. Results： No obvious toxic reaction was found after the mice received intragastric administration of Tong Nao Shu Mai pill. The maximum administration dosage was 67.9g/kg within one day, which was 136 times of human dosage; Tong Nao Shu Mai pill in the low and high dosage could lower the blood and plasma viscosity of the rat model of blood-stasis pattern notably（P〈0.05） which were caused by adrenaline and ice water. Compared with model control group, the differences had statistical meaning（P〈0.05）. Tong Nao Shu Mai pill in the low and high dosage could inhibit whole blood platelet aggregation of ADP-induced rats obviously, and it could obviously decrease one, six resistance values, the difference showed statistical meaning compared with the model control group（P〈0.05）. Conclusion： It＇s safe to take Tong Nao Shu Mai pill orally in large dose within one day, which could obviously decrease whole blood and plasma viscosity, inhibit platelet aggregation, promote platelet depolymerization and improve abnormal hemorheology.