腺苷脱氨酶1在肺腺癌组织的表达及其与预后的关系

Expression of adenosine deaminase acting on RNA 1 in lung adenocarcinoma and its correlation with prognosis

目的探讨腺苷脱氨酶l（ADAR1）在肺腺癌中的表达及其临床意义。方法应用免疫组织化学染色检测48例肺腺癌患者肿瘤组织与癌旁正常组织中ADAR1蛋白表达水平。应用实时定量反转录聚合酶链反应（RT—qPCR）检测肺腺癌患者肿瘤组织与癌旁正常组织中ADAR1基因表达水平。结果肺腺癌组织中ADAR1蛋白阳性表达率显著高于癌旁正常组织（60．4％比20．8％，P〈0．05）。肺腺癌组织中ADAR1基因表达水平显著高于癌旁正常组织（1．58比1．19，P〈0．05）。肺腺癌组织中ADAR1蛋白阳性表达率与肿瘤TNM分期（X2=7．779，P〈0．05）和淋巴结转移（X2=4．516，P〈0．05）密切相关。Kaplan—Meier生存分析显示，ADAR1高表达组患者的总生存期（OS）较低表达组明显缩短，其中ADAR1高表达组（29例）中位生存时间为27个月；低表达组（19例）中位生存时间为56个月，差异有统计学意义（X2=8．916，P〈0．01）。Cox比例风险回归模型分析结果显示，ADAR1蛋白表达水平、TNM分期是影响预后的独立因素（P〈0．05）。结论ADAR1在肺腺癌中高表达与患者预后差密切相关。

Objective To investigate the expression of adenosine deaminase acting on RNA 1 （ADAR1） and its correlation with clinicopathological characteristics and prognosis in lung adenocarcino- ma. Methods The expression of ADAR1 protein in lung adenocarcinoma tissues （ n = 48） and adjacent normal lung tissues （n = 48） was detected by immunohistochemistry. Real - time quantitative polymerase chain reaction （ RT - qPCR） was applied to detect the expression of ADAR1 mRNA in fresh lung adenocar- cinoma tissues and adjacent normal lung tissues. Correlation of the expression of ADAR1 with clinicopatho- logical characteristics and overall survival of lung adenocarcinoma patients was analyzed. Results The ex- pression of ADAR1 protein was significantly higher in lung adenocarcinoma tissues than in adjacent normal lung tissues （60. 4% vs. 20. 8%, P 〈 0. 05）. The expression of ADAR1 mRNA was significantly higher in lung adenocarcinoma tissues than in adjacent normal lung tissues （1.58 vs. 1.19, P 〈 0. 05）. The ex- pression of ADAR1 protein was significantly correlated with TNM stage （X2 = 7. 779, P 〈 0. 05） and lymph node metastasis （X2 =4. 516, P 〈0. 05）. The median overall survival time was longer in the low ADAR1 expression group than in the high expression group （56 months vs. 27 months, P 〈 0. 05） by Kaplan - Meier survival analysis. The cox multivariate analysis showed that ADAR1 expression and TNM stage were independent prognostic factors （P 〈 0. 05）. Conclusion ADAR1 is overexpressed in lung adenocarcino- ma. High expression of ADAR1 correlates with poor survival in lung adenocarcinoma.