星形细胞上调基因-1及细胞核增殖抗原在食管鳞癌组织中的表达及临床意义

The expression and clinical significance of astrocyte elevated gene - 1 and proliferation cell nuclear antigen in esophageal squamous carcinoma

目的探讨星形细胞上调基因-1（AEG-1）及细胞核增殖抗原（Ki-67）在食管鳞癌中的表达和临床意义。方法采用免疫组织化学方法检测56例食管鳞癌组织和相对应的56例正常食管组织中AEG-1及Ki-67蛋白的表达，并分析其表达与食管鳞癌临床病理学参数之间的关系。结果免疫组织化学结果显示AEG．1在食管鳞癌组织中阳性表达率（73．21％）显著高于正常组织（5．36％），两者表达差异有统计学意义（，=54．053，P〈0．01）。AEG-1表达与患者的性别和年龄无明显相关（P〉0．05），但与组织学分级、浸润深度、淋巴结转移和TNM分期均密切相关（P〈0．05）。Ki-67在食管鳞癌组织中阳性表达率（67．86％）显著高于正常食管黏膜组织（3．57％），两者表达差异有统计学意义（，=50．400，P〈0．01）。Ki-67表达与患者的性别和年龄无关（P〉0．05），但与组织学分级、浸润深度、淋巴结转移和TNM分期均密切相关（P〈0．05）。AEG—l和Ki-67表达的相关性分析结果表明，AEG-1及Ki-67在食管鳞癌组织中的表达强度呈正相关（r=0．792，P〈0．01）。结论AEG-1和Ki-67在食管鳞癌组织中的高表达可能与食管鳞癌的发生、发展及浸润转移密切相关。

Objective Our research attempts to study the expression and clinical significance of astrocyte elevated gene - 1 （ AEG - 1 ） and proliferation cell nuclear antigen （ Ki - 67 ） on human esopha- geal squamous cell carcinoma, The findings may provide the theoretical basis for molecular target therapy of esophageal squamous cell carcinoma. Methods Expression of AEG - 1 protein and Ki - 67 protein was detected in 56 cases of esophageal squamous cell carcinoma tissues and paired normal esophageal epithelial tissues by immunohistochemistry method, and the relationship between AEG - 1 protein and Ki - 67 protein expression and elinicopathologieal features of esophageal squamous cell carcinoma was investigated. Re- suits The results of immunohistoehemistry in that positive expression rate of AEG - 1 protein in esophage- al squamous cell carcinoma tissues （73.21% ） was significantly higher than that in normal esophageal epi- thelial tissues （5.36%） , with a significant difference between two groups （2＂2 = 54. 053, P 〈 0. 01 ）. AEG - 1 protein expression was not associated with patients＇ sex and age （ P 〉 0. 05 ）, but closely related to histogieal grade, invasive depth, lymph node metastasis and TNM staging （ P 〈 0. 05 ）. The results of immunohistoehemistry demonstrated that positive expression rate of Ki -67 protein in esophageal squamous cell carcinoma tissues （67.86%） was significantly higher than that in normal esophageal epithelial tissues （ 3.57% ） , with a significant difference between two groups （X2 = 50. 400, P 〈 0. 01 ）. Ki - 67 protein ex- pression was not associated with patients＇ sex and age （ P 〉 0. 05 ）, but closely related to histogical grade, invasive depth, lymph node metastasis and TNM staging （P 〈 0. 05）. The results of correlation analysis of AEG - 1 protein and Ki - 67 protein expressions demonstrated that the expression of AEG - 1 protein and Ki - 67 protein in esophageal squamous cell carcinoma tissues displayed positive correlation （ r = 0. 792, P 〈 0. 01 ）. Conclusion Overexpressions of AEG - 1 protein and Ki - 67 protein in esophageal squamous cell carcinoma tissues may be closely associated with the occurrence, development and progression of e- sophageal squamous cell carcinoma.