心房颤动相关微小RNA的筛选及临床意义

Screening of atrial fibrillation - related microRNA and clinical significance

目的筛选心房颤动（简称房颤）相关微小RNA（miRNA，miR），寻找潜在的干预靶点。方法随机选择我院2014年6月至2015年6月期间行瓣膜置换手术的风湿性心脏瓣膜病患者18例。分为两组：窦性心律组8例，房颤组10例，术中获取右心耳组织。利用miRNA芯片获得两组右心耳组织的miRNA表达谱信息，通过组间对比分析，从表达谱中筛选出差异表达的miRNA，并用实时定量反转录聚合酶链反应（RT-qPCR）进一步验证。结果芯片结果显示：相对于窦性心律组，共有27个miRNA在房颤组差异表达，其中表达上调12个，表达下调15个（P〈0．05）。依据设定的进一步筛选条件，我们从中选择miR-30c进行RT—qPCR验证。RT—qPCR结果显示：相对于窦性心律组，miR-30c在房颤组中表达下调（P〈0．05），与芯片结果一致。结论miR-30c的差异表达与房颤相关，可能参与房颤机制的调控。

Objective To screen atrial fibrillation -related microRNA （miRNA, miR） to identify potential targets for intervention. Methods We randomly selected 18 patients with rheumatic heart valve disease undergoing valve replacement surgery from June 2014 to June 2015 in our hospital. All patients were divided into two groups： sinus rhythm group （n =8）, and atrial fibrillation group （n = 10）. The right atrial appendage tissues were obtained at the time of surgery. The miRNA microarray was used to create miRNA expression profi/e of the right atrial appendage tissue. Then, the differentially expressed miRNA was screened through comparative analysis between two groups. Finally, real - time quantitative reverse transcriptase - polymerase chain reaction （ RT - qPCR） was used for further validation. Results Microar- ray results showed that 27 miRNAs were differentially expressed in the atrial fibrillation group relative to the sinus rhythm group： 12 miRNAs were upregulated, and the rest 15 were downregulated （P 〈0. 05）. Ac- cording to the screening criteria, we choose miR - 30c for RT - qPCR validation. RT - qPCR results showed that the expression of miR - 30c was downregulated in atrial fibrillation group relative to the sinus rhythm group （ P 〈 0. 05 ）, consistent with the microarray results. Conclusion The differential expression of miR - 30e was associated with atrial fibrillation and may be involved in regulating mechanism of atrial fi- brillation.