针刺治疗肠易激综合征的临床观察及其对调节性T细胞功能的影响

Effects of acupuncture via Tianshu and Zusanli point on the regulatory T cells for patients with IBS

目的观察针刺天枢穴与足三里穴对肠易激综合征(IBS)患者CD4+CD25+调节性T细胞(Treg)、CD8+CD28-调节性T细胞和TGF-β、IL-10的影响。方法将纳入的IBS 30例患者随机分成天枢组、足三里组合配伍组,分别予天枢穴、足三里穴和足三里穴+天枢穴针刺治疗7 d,另设置健康对照组10例。结果针刺治疗后与未治疗前IBS患者各项指标具有显著的差异性(P<0.01);健康组与配伍组之间无统计学意义(P>0.05);余各组除天枢组CD8+CD28-T(%)指标外,均与健康组有统计学差异(P<0.05);针刺组内部互相比较:与足三里组比较,除天枢组的CD4+CD25+T(%)指标无统计学差异外(P>0.05),余皆具有统计学意义(P<0.05);配伍组与天枢组相比,差异具有统计学意义(P<0.05)。结论针刺天枢和足三里能明显的调节Treg细胞百分比与TGF-β1和IL-10,从而改善机体的免疫机能。

Objective To observe effects of Tianshu point and Zusanli point on IBS patients,and the levels of CD4＋ CD25-Treg,CD8 ＋ CD28-Treg,TGF-β and IL-10 in plasma.Methods 30 patients were qualified for inclusion,with 10 in each group randomly,included Tianshu group and Zusanli group and Tianshu ＋ Zusanli group.Besides,a normal group with 10 patients were included.The acupuncture group were given electroacupuncture for 7 days,while the normal group was done nothing.Results Compared with before treatment after treatment,the detection index had a statistically significant difference（P0.01）;No statistical significance between healthy group and experimental group（P0.05）;Compared with Zusanli group,except for the CD8＋CD28-T（%）of Tianshu group（P0.05）,other groups were significantly different（P0.05）; Compared with Tianshu group and Tianshu ＋ Zusanli group,it had a significant difference（P0.05）.Conclusion Electroacupuncture the Tianshu and Zusanli can regulate the CD4＋ CD25-Treg、CD8＋ CD28-Treg、TGF-β and IL-10 in plasma obviously which can finally enhance body ＇ s immune.