汽化过氧化氢对不同材料表面的消毒效果比较

Comparison of disinfection effect of different material surface after using hydrogen peroxide vapor

目的研究汽化过氧化氢对不同材料物体表面受污染后的消毒效果,为对不同内环境的室内空间的消毒控制提供参考。方法采用5种材料制备染菌载体,定量杀灭枯草杆菌黑色变种芽孢和嗜热脂肪杆菌芽孢,观察过氧化氢气体在不同时间内对5种材质菌片的消毒效果。结果在30 m3密闭空间内用汽化过氧化氢对菌片进行10、20、30、40、60 min处理。以枯草杆菌黑色变种(ATCC 9372)芽孢为试验菌时,作用30 min对塑料载体上芽孢的杀灭对数值>6.00;作用60 min对棉布、印刷纸、不锈钢和玻璃载体上芽孢的杀灭对数值均>6.00。以嗜热脂肪杆菌(ATCC 7953)芽孢为试验菌时,作用30 min对不锈钢、玻璃和塑料载体上芽孢的杀灭对数值均>6.00,作用60 min对棉布和印刷纸载体上芽孢的杀灭对数值均>6.00。结论在设定的程序下,汽化过氧化氢对表面无孔无渗透材料的消毒效果显著好于表面多孔渗透性材料。

Objective To reaserch the disinfection effect of hydrogen peroxide vapor in different material surface contaminated with pathogens,and to provide the basis for disinfection and sterilization in interior space with a variety of environment. Methods Five materials were used to prepare for biological indicator,the spore of Bacillus subtilis（ ATCC 9372） and Bacillus stearoshermophilus（ ATCC 7953） were used in quantitative germicidal test by hydrogen peroxide vapor in different time. Results After sterilization in the 30 m3 confined space in 10 min,20 min,30 min,40 min and 60 min,The killing logarithms value of the spore of Bacillus subtilis on plastic carrier for 30 min was above 6. 00. The killing logarithms value of the spore of Bacillus subtilis on the carrier of cotton,printing paper,stainless steel and glass for 60 min were all above 6. 00.As for the spore of Bacillus stearoshermophilus,the killing logarithms of the spore on the carrier of stainless steel,glass and plastic for 30 min were all above 6. 00. The killing logarithms of the spore on the carrier of cotton and printing paper for60 min were both above 6. 00. Conclusion The disinfection effect of hydrogen peroxide vapor to nonporous and non- permeable materials was significantly better than porous and permeable materials.