一种复方胍类消毒剂性能研究

Study on properties of a compound guanidine disinfectant

目的了解一种含聚六亚甲基胍和乙醇的消毒液性能。方法采用悬液定量杀菌试验、现场试验和毒理试验方法对其进行观察。结果以含4 700 mg/L的聚六亚甲基胍和体积分数为52.30%的乙醇组成复方手消毒液作用0.5 min,对悬液内的大肠杆菌、金黄色葡萄球菌和铜绿假单胞菌的平均杀灭对数值均>5.00;作用0.5 min对悬液内的白色念珠菌杀灭对数值>4.00;对手上自然菌作用1 min的杀灭对数平均值为1.28;在37℃温度下密闭90 d,消毒液中的聚六亚甲基胍和乙醇含量下降率分别为4.64%和6.55%。其原液急性经口毒性LD50>5 000mg/(kg·bw),对家兔皮肤无刺激性,对小豚鼠皮肤无致变态反应作用,小鼠骨髓多染红细胞微核试验结果为阴性。结论该复方胍类手消毒液具有良好的杀菌效果与稳定性,属于实际无毒级、无刺激性的消毒剂,具有良好应用价值。

Objective To study the properties of a disinfectant containing polyhexamethylene guanidine and ethanol as the main germicidal ingredients. Methods Suspension quantitative germicidal test,simulated field disinfection test and animal toxicity test were performed to observe the germicidal efficacy and the toxicity. Results The killing logarithm values of Escherichia coli,Staphylococcus aureus and Pseudomonas aeruginosa exposed to the compound hand disinfection solution containing 4 700 mg / L polyhexamethylene guanidine and 52. 30% ethanol for 0. 5 min were all above 5. 00. The killing logarithm value of Candida albicans exposed for 0. 5 min was above 4. 00. The killing logarithm value of the natural bacteria on hands was 1. 28 after wiping disinfection with the disinfection solution for 1 min. The concentration of polyhexamethylene guanidine and ethanol reduced 4. 64% and 6. 55% respectively after being kept in 37 ℃ for 90. The mouse acute oral LD50 of this disinfectant was 〉5 000 mg（ kg·bw） and mouse bone marrow polychromatic erythrocyte micronucleus test was negative. Moreover,the hand disinfection solution was not irritate to rabbit skin,as well as had no allergic response to guinea-pig skin. Conclusion This compound guanidine hand disinfectant has a high value of practical application because of its good germicidal efficacy on bacteria vegetative and good stability,while it has no irritation to animal skin and no toxicity.