ARMc8在非小细胞肺癌的表达及意义

The expression and significance of ARMc8 in non-small cell lung cancer

目的探讨ARM重复蛋白8(ARMc8)在非小细胞肺癌(NSCLC)中的表达,分析其与临床病理学特点及预后的关系。方法采用免疫组化SP法检测153例NSCLC中ARMc8的表达和定位,Western blot检测ARMc8在正常支气管上皮细胞HBE和肺癌细胞A549、H1299和SPC中的表达情况。结果 NSCLC和癌旁肺组织中2ARMc8的阳性率分别为70.59%(108/153)和12.42%(19/153),两组相比具有显著性差异(χ=106.612,<0.001)。ARMc8在肺癌细胞中的表达水平显著高于HBE细胞(<0.01);ARMc8的高表达与患者的TNM分期(=0.021),淋巴结转移(=0.002)和预后(<0.001)密切相关;COX分析表明ARMc8阳性表达是影响NSCLC患者预后的独立危险因素(=0.014和=0.015)。结论 ARMc8促进NSCLC恶性进展和不良预后,是潜在的治疗靶点和预后判定指标。

Objective To explore the expression of armadillo-repeat-containing protein 8 （ARMc8） and its correlation with clinicopathological parametors and prognosis in non-small cell lung cancer （NSCLC）. Methods SP immunohistochemistry was used to detect the expression of ARMc8 in 153 cases of NSCLC（67cases of squamous cell carcinoma, 70 cases of adenocarcinoma, 10 cases of large cell carcinoma and 6 cases of adenosquamous carcinoma） and their adjacent noncancerous lung tissues; and Western blot was used to detect the expression of ARMc8 in HBE（normal bronchial epithelial cell line） and three lung cancer cell lines A549, H1299 and SPC. Results The positive expression ratio of ARMc8 in lung cancer tissues and adjacent lung tissues were 70.59% （ 108/153 ） and 12.42% （ 19/153 ） respectively with significant difference between two groups （ x^2=106-612, p〈0.001）. The expression level of ARMc8 in three lung cancer cells was significantly higher than that in HBE （p〈0.01）, The overexpression of ARMc8 in NSCLC was relevant to TNM stage （ p =0.021） and lymph node metastasis （ p=0.002） of lung cancer patients, and was an independent prognostic factor for lung cancer patients （ p=0.014 and p=0.015）. Conclusion ARMc8 promotes the malignant progression and poor prognosis of NSCLC, and might be a potential therapeutic target and prognosis indicator.